传统发酵大头菜中微生物群落结构的动态变化及益生特性研究

项目名称： 传统发酵大头菜中微生物群落结构的动态变化及益生特性研究

项目编号： No.31501456

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 吴进菊

作者单位： 湖北文理学院

项目金额： 20万元

中文摘要： 湖北襄阳大头菜是我国四大名腌菜之一，是国家地理标志产品，历史悠久，发酵过程中蕴藏了丰富的微生物资源。目前针对传统发酵大头菜中微生物方面的研究甚少，且采用的都是传统纯培养的方法，很难全面揭示发酵中微生物的多样性和群落结构变化。本项目采用Illumina Miseq高通量测序技术对传统发酵大头菜中的微生物多样性和菌群结构动态变化进行分析，结合纯培养方法和现代分子生物学技术对大头菜中的优势微生物进行分离、纯化和鉴定，构建发酵大头菜菌种资源库，并进行耐酸性、耐胆盐性、抗氧化特性和降低胆固醇特性等益生特性的研究，以筛选获得特性良好的益生菌株。. 通过本项目的研究，将从基因水平上全面揭示传统发酵大头菜中微生物的多样性和群落结构的动态变化，建立发酵大头菜菌种资源库，开发自然微生物资源，为今后大头菜的工业化、标准化生产和益生菌的开发利用提供一定的研究基础。

中文关键词： 菌群变化；多样性；菌群结构

英文摘要： Hubei Xiangyang kohlrabi was one of the China's four famous pickles and it was the national product of geographical indication. It had a long history, and contained the abundant resources of microorganisms during the fermentation process. At present, there were a little research on the microorganisms of traditional fermented kohlrabi, and general traditional culture methods were used. Therefore it was difficult to fully reveal the diversity and community structure changes of microorganisms in microbial fermentation. Illumina Miseq high-throughput sequencing technology was used to analyse the microbial diversity and structure of dynamic flora changes in traditional fermented kohlrabi. Pure culture methods were combined with modern molecular biology technique to separate, purify and identify the dominant microorganisms, and build microorganisms repository of fermented kohlrabi. To screening out probiotics of good properties, acid resistance, bile salt resistance, antioxidant properties and lower cholesterol were analysed. . Through this project research, it will fully reveal the diversity and community structure changes of microorganisms of traditional fermented kohlrabi on gene level, build microorganisms repository and develop natural microbial resources. It will provide a research basis for the development and utilization of probiotics and the next kohlrabi industrial and standardized production.

英文关键词： microbial population change ;diversity;community structure