项目名称: 水稻谷胱甘肽过氧化物基因家族的功能研究
项目编号: No.31300227
项目类型: 青年科学基金项目
立项/批准年度: 2014
项目学科: 生物科学
项目作者: 王欣
作者单位: 武汉大学
项目金额: 23万元
中文摘要: 相对动物而言,高等植物谷胱甘肽过氧化物酶(Glutathione peroxidases, GPXs)的功能远不止是一种抗氧化酶那么简单,它们更多的参与了细胞氧化还原信号传递以及植物生长发育的复杂调控过程,其确切的生理功能尚不明确。深入研究模式植物水稻OsGPXs基因家族的功能,可以为将来通过遗传操作等手段精细调控体内GPX表达水平以提高农作物抗逆境胁迫能力,促进光合生理的品种改良奠定基础。本项目首先从5个OsGPX基因家族成员转录表达模式、蛋白活性以及亚细胞定位分析作为切入点,然后利用转录激活子样效应因子核酸酶靶向敲除技术,创建水稻叶绿体型OsGpx4突变体,对其基因功能以及抗氧化机制做进一步探讨。在此基础上创建雌二醇诱导型启动子调控下的OsGPX4过表达植株,分析它们与野生型植株在逆境胁迫条件下生理、生化等各方面指标的差异,期望筛选到抗逆性提高的转基因株系。
中文关键词: 谷胱甘肽过氧化物酶;氧化胁迫;水稻;活性氧;硫氧还蛋白
英文摘要: Compared to animals, glutathione peroxidases (GPXs) are more than simple antioxidant scavengers in high plants; they may also act as redox sensors in signal transduction pathways and participate in the regulation of plant growth and development process. So far, the exact physiological roles of plant GPXs are not clearly known. Thus, understanding the function of OsGPXs gene family in rice, a model plant in molecular biology, will help us to finely manipulate the expression level of OsGPX to enhance the tolerance of environmental stress by genetic eginerering in future. In this study, we first analyze the transcriptional expression patterns, enzyme activities and subcelluar localiztion of OsGPXs. Secondly, the function of chloroplastic OsGpx4 and the antioxidant mechanism are analysed in OsGpx4 knockout mutant that is obtained by use of transcription activator-like effector nucleases (TALEN) technique platform. Further, trangenic rice are generated by expressing OsGpx4 that is positioned downstream of the β-estradiol-inducible promoter. The physiological or biochemical index of the transgenic lines and wild type are analyzed in order to obtain enhanced tolerance lines.
英文关键词: Glutathione peroxidase;Oxidative stress;Rice;Reactive oxygen species;Thioredoxin