经血来源的间充质干细胞对乳腺癌的趋向作用及其内在机制研究

项目名称： 经血来源的间充质干细胞对乳腺癌的趋向作用及其内在机制研究

项目编号： No.81201089

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 影像医学与生物医学工程

项目作者： 王浩浩

作者单位： 浙江大学

项目金额： 23万元

中文摘要： 人经血来源的间充质干细胞（MenSCs），是近年发现的新型成体干细胞，可从女性经血中方便的分离获取和扩增，具有可塑性/低免疫原性以及极低致瘤性等特点。就MenSCs的内在本质研究和应用研究而言，MenSCs对肿瘤的趋向性及内在的作用机理都是一个关键问题。本课题组前期动物实验结果证实MenSCs具有损伤趋向性。因而，设想其对恶性肿瘤组织也存在定向迁移的特性，拟通过研究多种乳腺癌细胞系体外对MenSCs的趋向性，检测参与该过程的各种旁分泌途径和受体配体途径，阐明内在的作用机理；建立多种乳腺癌细胞系的裸鼠动物模型，采用新型超顺磁性氧化铁颗粒体外标记后体内MRI动态示踪，验证确切的效果和机制。从而为进一步认识MenSCs以及临床应用打下坚实的基础。

中文关键词： 间充质干细胞；经血；迁移；恶性肿瘤；

英文摘要： One source of blood mesenchymal stem cells (MenSCs), is a new type of adult stem cells found in recent years, women in the blood from the separation of convenient access and expansion, have plasticity/low immunogenicity and very low tumorigenicity etc. Characteristics. MenSCs is the inner nature of research and application in research, MenSCs to the tumor and internal mechanism of the tendency is a key problem. In the course of the animal experiment results show MenSCs has the tendency of damage. So, imagine the malignant tumor tissue also has the characteristics of directional migration, proposed by the variety of breast cancer cell lines of research in the tendency of MenSCs, participate in the process of testing the paracrine way and receptor ligands way, expounds the inner mechanism; Establish a breast cancer cell lines LuoShu of animal model, using new superparamagnetic iron oxide particles in vitro after mark in MRI dynamic tracer, verify the exact effect and mechanism. And so as to further understanding MenSCs and clinical application and lay a solid foundation.

英文关键词： Mesenchymal stem cell；Menstrual blood；migration；malignant tumor；