能量转移型肼的上转换纳米复合比率荧光探针的制备及荧光成像

项目名称： 能量转移型肼的上转换纳米复合比率荧光探针的制备及荧光成像

项目编号： No.U1504203

项目类型： 联合基金项目

立项/批准年度： 2016

项目学科： 环境科学、安全科学

项目作者： 李占先

作者单位： 郑州大学

项目金额： 27万元

中文摘要： 作为一种重要的化学试剂，肼被广泛应用于药物、农药、防腐剂和纺织染料等化工业，同时肼又是一种高毒性物质并且很容易被人体吸收。随着人类对健康和环境污染的日益关注，有关肼的检测研究越来越受到人们的广泛关注。.本课题拟以1,8-萘酰亚胺为母体，通过明确的分子设计与合成，揭示结构与性质的内在联系，得到对肼具有高灵敏性和高选择性的有机分子荧光探针。以此为基础，制备稀土上转换纳米粒子，使其发射光谱能够与分子探针的吸收光谱重叠。将有机分子荧光探针与稀土上转换纳米粒子进行键合，得到有机-无机复合纳米荧光探针，研究有机分子结构、纳米颗粒表面修饰方式和纳米粒子大小等对能量转移效率的影响，阐明复合纳米荧光探针与肼在细胞或生物组织内部相互作用的化学机制，最终得到近红外光激发、荧光共振能量转移型肼的高灵敏性、高选择性比率复合纳米荧光探针。

中文关键词： 细胞成像；构效关系；荧光标记

英文摘要： As an important chemical reagent, hydrazine is widely used in pharmaceuticals, pesticides, corrosion inhibitors and textile dyes in various chemical industries. On the other hand, hydrazine is highly toxic and could be readily absorbed. With the increasing concern for health and the environmental pollution, more and more people pay attention to the study of hydrazine detection..In this project, through the clear design and synthesis of molecules, we try to clarify the inner relationship between structure and properties, and obtain several 1,8-naphthalimide-based molecular fluorescent probes towards hydrazine. Based on the above results, we want to prepare rare-earth upcoversion nanophosphores, whose emission spectra overlap the absorption spectra of the obtained small molecular fluorescent probes. We try to modify upcoversion nanophosphores by molecular fluorescent probes, summarize the influence factors of the organic small molecular structure, surface modification methods and size of nanoparticles on the energy transfer efficiency of the composite materials, and clarify the interaction mechanism of composite nanometer fluorescent probes with hydrazine in the intra cell or biological tissues. We will finally obtain near-infrared light-excited and luminescent resonance energy transfer-based nanocomposites as excellent selective and sensitive ratiometric fluorescent probes for hydrazine.

英文关键词： Cell imaging;Structure-activity relationship;Fluorescence labeling