血流环境中白细胞介导纳米药物载体与肿瘤细胞的靶向结合研究

项目名称： 血流环境中白细胞介导纳米药物载体与肿瘤细胞的靶向结合研究

项目编号： No.11302129

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 傅怡

作者单位： 上海交通大学

项目金额： 26万元

中文摘要： 临床数据表明因癌症死亡的病人90%归结于肿瘤转移。血液中肿瘤细胞的微转移是形成继发性肿瘤的先决条件，各种炎症细胞，如中性粒细胞、淋巴细胞和单核细胞等，都与肿瘤及其转移有着密不可分的联系。细胞力学研究已揭示了白细胞借助靶向结合在肿瘤细胞通过血液循环的转移中起介导作用。同样的机理能否用于将抗癌药物微纳米粒子有效输送到病灶部位与肿瘤细胞结合，成为抗癌药物靶向治疗的一个热点。本项目拟在前者研究成果的基础上，进行流动状态下白细胞介导微纳米药物粒子与肿瘤细胞的靶向结合研究：1）通过体外实验方法，探索白细胞介导纳米药物载体与肿瘤细胞靶向结合的可行性和效率；2）定量研究白细胞介导靶向微纳米粒子与肿瘤细胞结合的靶标、流动条件及效率。目标为微纳米靶向药物载体的设计，提高其输送并杀灭肿瘤细胞的效率提供实验依据。

中文关键词： 白细胞；肿瘤细胞；纳米药物载体；血流；黏附

英文摘要： Clinical data shows that about 90% death cases are due to tumor metastasis. The metastassis of tumor cells may lead to the formation of secondary tumor, Studies demostrated that inflammatory cells, such as leukocytes, lymphocytes and monocytes, have close connections to tumor and tumor metastassis. Cell mechanics studies have indicated that white blood cells could facilitate the metastasis of tumor cells in blood flow by targeted adhesion. However, whether the similar mechanism could be applied to anti-tumor nanoparticle carriers transportation to tumor focus is rarely known, which is of great interest and remains challenging. Based on previous studies we intend to take advantage of those white blood cells to facilitate nanoparticle drug carriers targeting tumor cells under blood flow in this proposal, 1) We will first explore the feasibility that white blood cells may act as "Trojan" to help nanoparticle drug carriers bind to tumor cells by in vitro experiment; 2) Furthermore, we will quantify the target molecules, flow conditions and efficiency in the process that white blood cells facilitate adhesion between nanoparticle carriers and tumor cells. We aim to provide promising experimental evidence for tumor-targeted nanoparticle carriers design and improve the efficiency of drug delivery.

英文关键词： white blood cells；tumor cells；nanoparticle drug carriers；blood flow；adhesion