microRNA参与调控新生儿支气管肺发育不良的分子机制研究

项目名称： microRNA参与调控新生儿支气管肺发育不良的分子机制研究

项目编号： No.81070524

项目类型： 面上项目

立项/批准年度： 2011

项目学科： 生物科学

项目作者： 张小英

作者单位： 中国人民解放军北京军区总医院

项目金额： 10万元

中文摘要： 支气管肺发育不良（BPD）是儿童慢性肺疾患的主要病因，但其发病机制却不清楚。microRNA（miRNA）分子是一种长约21-25 碱基的非编码的单链小分子RNA，参与调控生物体内多个重要的生物学过程，包括肺发育和肺部疾病。本课题以miRNA 芯片为探索起点，利用动物模型（BPD 新生小鼠）和病人标本（BPD 患儿血浆、或肺组织）从中筛出与BPD 特异相关的miRNA，拟采用芯片技术、PCR、Western Blot、免疫组化、荧光酶报告实验、流式、动物模型等技术，从分子（过表达或干扰）、细胞（肺上皮细胞）和整体（BPD 新生小鼠）水平上进行不同层次的研究，探讨miRNA 在BPD 发病过程中（尤其是早期病变）的作用。

中文关键词： 新生儿；支气管肺发育不良；肺损伤；microRNA

英文摘要： Bronchopulmonary dysplasia (BPD) is a chronic lung disease manifested in preterm neonates, yet the underlying pathogenesis is not fully understood. MicroRNAs (miRNAs) are known as 21-25 nucleotides RNA molecules with strong functions in development, cellular differentiation, apoptosis, proliferation and migration. However, very little is known regarding the role of miRNAs in the progress of developmental lung diseases. In this proposal, microarrays were performed in BPD animal model to detect the special miRNAs that related with BPD, and to be validated in human blood samples and lung tissues samples.Furehermore, QRTPCR, WB, IHC, FACS were also carried out in the study. We have figured out a clue about the new mechanism of the development of BPD from the different levels in vitro(overexpression/silencing experiments) and in vivo (animal model).

英文关键词： newborn; bronchopulmonary dysplasia; lung injury; microRNA