土著产黄青霉浸出修复重金属重污染土壤机理研究

项目名称： 土著产黄青霉浸出修复重金属重污染土壤机理研究

项目编号： No.51474102

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 矿业工程

项目作者： 邓新辉

作者单位： 湖南工业大学

项目金额： 82万元

中文摘要： 铅锌冶炼废渣堆场土壤遭受重金属复合污染，急需发展清洁高效、成本低廉的冶炼废渣堆场土壤治理技术。项目组已从某大型冶炼厂冶炼废渣堆场重污染土壤中筛选到一株具重金属抗性和产酸能力的产黄青霉，该菌株对重金属含量高达12.62 g/kg重污染土壤中重金属的浸出量为323.2 mg/L，对严重重金属复合污染土壤具有良好的修复效果，但对其作用机理不明。本项目拟采用13C元素示踪代谢通量分析法和典范相关分析法，结合高效液相色谱、同步辐射X射线吸收光谱技术和核磁共振等分析手段，研究：1) 产黄青霉的产酸机制；2) 浸出修复动力学机制；3) 浸出修复重污染土壤工艺特征，揭示产黄青霉浸出修复重金属的作用机理。本项目研究旨在阐明重金属重污染土壤微生物修复的基础理论，形成土著产黄青霉浸出修复严重重金属复合污染土壤技术原型。

中文关键词： 土壤重金属复合污染；浸出修复；产黄青霉；土著微生物；动力学机制

英文摘要： A large amount of slags rich in kinds of heavy metal have been produced during Pb/Zn smelting process. The smelting slags were directly piled so that the soil was polluted severely by heavy metal at the Pb/Zn smelting slag site. Remediation on the soil at Pb/Zn smelting slag site is a focus in the world, especially in China. Developing of an environment friendly,simple to operate,quickly to achieve and cost effective remediation technology on heavy metal compound pollution in soil is necessary. A Penicillium chysogenum, which has heavy metal resistance and is able to produce organic acid has been isolated from the polluted soil on Pb/Zn smelting slag site in the preliminary study. The leaching amount of heavy metal using Penicillium chrysogenum is 323.2 mg/L from the polluted soil , in which heavy metal content is as high as 12.62g/kg,so the bioleaching remediation effect was good, but the bioleaching mechanism is unclear.The followings will be researched using 13C metabolic flux analysis method (13C-MFA) and canonical correlation analysis (CCoA) method combined with analytical tools such as HPLC, MS and NMR: 1) the mechanism and approach of producing organic acids by Penicillium chrysogenum under multidimensional environment; 2) the leaching dynamics mechanism of Penicillium chrysogenum; 3) the characterization of leaching technology for Penicillium chrysogenum. Then some results would be obtained : 1) the leaching mechanism would be revealed at the molecular level; 2) the leaching efficiency and amount would be increased; 3)the technological characteristic on bioleaching heavy metals from heavily polluted soil at the Pb/Zn smelting slag site would be clarified. The project aims to provide basic theory on bioleaching remediation of heavy metal heavily compound pollution in soil using microbiology and a bioleaching technical prototype for the soil at Pb/Zn smelting slag site using indigenous Penicillium chrysogenum would be achieved.

英文关键词： the compound pollution of heavy metal in soil;leaching remediation;Penicillium chrysogenum;indigenous microbiology;dynamics mechanism