盐碱土壤转Bt基因棉花外源蛋白表达量时空变化对抗虫性影响的研究

项目名称： 盐碱土壤转Bt基因棉花外源蛋白表达量时空变化对抗虫性影响的研究

项目编号： No.31501253

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 食品科学、农学基础与作物学

项目作者： 雒珺瑜

作者单位： 中国农业科学院棉花研究所

项目金额： 20万元

中文摘要： 棉花生长期外源蛋白表达量时空变化直接影响Bt棉抗虫性变化，进而影响棉花抵御害虫及外界逆境胁迫能力，最终影响棉花生长发育及产量品质形成。在粮棉争地矛盾日益突出，土壤盐渍化和次生盐渍化不断加剧的背景下，基于Bt棉向盐碱地大面积转移种植趋势，明确盐碱土壤Bt棉外源蛋白表达量时空变化规律及其与抗虫性的关系，采取耐盐碱栽培调控措施，增强棉花自身抵御外界逆境能力，提高棉花产量和品质是确保盐碱地棉花丰产丰收的迫切需要和关键因素之一。本项目以转Bt基因棉花为试验材料，以其亲本非转基因棉花为对照，从土壤盐分水平动态、外源蛋白表达量时空变化入手，辅以分析生化物质及相关酶活性，研究并阐明盐碱土壤转Bt基因棉花外源蛋白表达量时空变化对抗虫性影响的生理生态机制，解析盐碱地Bt棉抗虫不均匀和时空动态生产实践现象，为增强盐碱地Bt棉对害虫及外界逆境胁迫抵御能力采取适度栽培调控措施提供理论依据。

中文关键词： 盐碱土壤；；转Bt基因棉花；；外源蛋白表达量；；时空变化

英文摘要： During the growing period of cotton, temporal and spatial variation of foreign protein expression directly affects insect resistance of Bt-cotton, and then affects the resistance ability of cotton to insects and external adversity stress, and ultimately affects growth of cotton as well as yield and quality. Due to more and more soil is planted with food crop as well as salinization and secondary salinization of soli is increasingly severe, Bt-cotton is massively planted in saline-alkali soil. Under this circumstance, in order to ensure the bumper harvest of cotton in saline-alkali soil, it is the urgent need and one of the key factors to find out temporal and spatial variation rule of foreign protein expression of Bt-cotton and its relationship with insect resistance in saline-alkali soil, take saline-alkali-resistant cultivation techniques and enhance the resistance ability of cotton to the external adversity to improve yield and quality of cotton.Therefore, the Project, on the basis of dynamic salt state of the soil and temporal and spatial variation of foreign expression as well as analysis of biochemical substances and the related enzyme activity at the same time, takes Bt-cotton as experimental material and its parental non-transgene cotton as the comparing material to study and explain physiological and biochemical mechanisms of influence that temporal and spatial variation of foreign protein expression of cotton exerted on insect resistance, and analyze the production practice under insect-resistant nonuniformity and dynamic space-time condition, which provides a theoretical guidance for taking cultivation regulation technology to enhance the resistance ability of Bt-cotton to insects and external adversity stress during the growing period in saline-alkali soil.

英文关键词： saline-alkali soil;transgenic Bt cotton;expression of Bt protein;temporal and spatial variation