无蓝紫光负效应的成像治疗一体化NaYF4:Yb3+, Tm3+上转换纳米载体构建

项目名称： 无蓝紫光负效应的成像治疗一体化NaYF4:Yb3+, Tm3+上转换纳米载体构建

项目编号： No.11204021

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 物理学I

项目作者： 宋凯

作者单位： 长春师范大学

项目金额： 25万元

中文摘要： 发光上转换纳米晶（UCNPs）由于有效激发位于红外区，对生物组织穿透深、光损伤小、背景荧光低等优点，近年来成为荧光标记和光动力治疗方面的研究热点。其中NaYF4:Yb3+, Tm3+ UCNPs在800 nm的近红外光实现深层肿瘤成像颇受关注。但这种方式有个不可避免的缺点是额外出现的蓝紫光会伤害肿瘤周围正常的组织。为此，我们设计了一个"NaYF4:Yb3+, Tm3+ UCNPs-抗肿瘤药物阿霉素-靶向分子适配子"双荧光共振能量传递体系的复合体。在Tm3+离子800 nm成像荧光不受影响的前提下，蓝紫光共振传递给阿霉素而被有效抑制，同时阿霉素的发光又传递给适配子排除对成像荧光信号的干扰。当这个构建靶向到肿瘤时，适配子构象改变迫使阿霉素释放，双荧光共振能量传递体系得以瓦解。进而恢复强度的蓝紫光协同阿霉素杀伤肿瘤。

中文关键词： 上转换纳米晶；光声成像；荧光能量共振传递；；

英文摘要： Luminescent upconversion nanocrystals (UCNPs) has several advantages, including effectively excited in the infrared region, absence of photo-damage to living organisms, very low auto-?uorescence and high light penetration depth in biological tissues. It has become research focus for fluorescence labeling and photodynamic therapy research in recent years. A typical case explored the use of the near-infrared optical imaging in the 800 nm of NaYF4: Yb3+, Tm3+ UCNPs for deep tumors. But there is an inevitable drawback is the extra blue-violet light damage will hurt the normal tissue around tumor. To address this problem, we have constructed a dual fluorescence resonance energy transfer consisted of "NaYF4:Yb3+, Tm3+ UCNPs-anticancer doxorubicin-targeted aptamer" complex. In the premise of the 800 nm fluorescence imaging of the Tm3+ is not affected, blue-violet owing to resonance passed to doxorubicin is quenched while simultaneously the fluorescence of doxorubicin transferred to aptamer resulting in eliminated imaging fluorescence signal interference. After the complex is taken up by targeted cancer cells, doxorubicin is gradually released from the conjugate because aptamer conformational changed, which induces the activation of blue-violet light and doxorubicin to synergisticly destroy tumor.

英文关键词： upconversion nanoparticles；photoacoustic imaging；fluorescence resonance energy transfer；；