HO-1通过p38信号通路调控高温诱导的牛卵巢颗粒细胞凋亡机制的研究

项目名称： HO-1通过p38信号通路调控高温诱导的牛卵巢颗粒细胞凋亡机制的研究

项目编号： No.31501955

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 畜牧学与草地科学

项目作者： 李莲

作者单位： 南京农业大学

项目金额： 19万元

中文摘要： 由高温诱导产生的血红素加氧酶1（HO-1）又称热休克蛋白32，是一种具有抗凋亡和细胞保护作用的抗氧化防御酶，但其对高温诱导的牛卵泡颗粒细胞凋亡的作用机制尚不清楚。基于研究组前期发现HO-1是通过p38信号通路对大鼠卵巢发挥保护作用，故本研究拟首先通过在体实验研究高温及非高温环境牛卵巢HO-1基因及其下游p38信号通路表达的变化，进而通过离体实验体外培养牛卵泡颗粒细胞，并通过激活或阻抑HO-1基因研究高温应激状态下卵泡颗粒细胞HO-1基因及其下游p38信号通路表达变化，同时进一步分析HO-1代谢产物信号分子CO对p38信号通路及雌激素分泌的调节，旨在揭示HO-1对高温诱导的牛卵巢颗粒细胞凋亡的作用机制，为高温应激环境下奶牛颗粒细胞凋亡的诱因和机制提供理论支撑。

中文关键词： 奶牛；高温；血红素加氧酶1；颗粒细胞；凋亡

英文摘要： Hemeoxygenase 1 (HO-1/HSP32) play an anti-apoptotic and cytoprotective effects in various cells, but little is known about its definitive function in granulosa cells of cows. We have found the protective role of HO-1 for ovary of rat via p38 signaling pathway. To clarify the underlying role of HO-1 in granulosa cells of cows , HO-1 gene expression and related signals involved in p38 signaling pathway in ovary and culture granulosa cells were analysed; expression of HO-1 was silenced or activated in the granulosa cells using small interfering RNAs (siRNAs) or HO-1 inducer to further verify the functional role of HO-1 through p38 signaling pathway mediated in granulosa cells; meanwhile, the levels of gas signaling molecule and E2 in cultured media were determined to further clarify the mechanism of HO-1 on granulosa cells of cows via p38 signaling pathway. The research seeks to provide a theoretical basis for possible causes and regulation mechanism of granulosa cells apoptosis in summer environment.

英文关键词： cow;heat;hemeoxygenase-1;granulosa cells;apoptosis