硅酸钠提高马铃薯黑痣病抗性相关基因功能分析

项目名称： 硅酸钠提高马铃薯黑痣病抗性相关基因功能分析

项目编号： No.31460468

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 张笑宇

作者单位： 内蒙古农业大学

项目金额： 47万元

中文摘要： 马铃薯黑痣病（Rhizoctonia solani）是世界马铃薯生产上的一种重要病害。在内蒙古马铃薯种植区危害严重，目前缺乏有效的防治措施。硅及硅酸盐类能提高植物抗病性已在很多病害中得到证实。前期经室内和田间试验研究，硅酸钠能提高马铃薯地下茎、块茎对黑痣病的抗性，且能增加产量并改善品质。本课题重点研究硅酸钠提高马铃薯黑痣病抗性的分子机制，即通过新一代高通量Illumina测序及生物信息学分析，筛选出硅酸钠诱导的马铃薯抗病相关基因，再利用Real-Time PCR技术分析其时空表达和进行功能预测，并用RACE技术克隆抗病相关基因的全长cDNA进行表达产物分析，确定目标功能基因，再通过转基因超量表达进行候选基因的功能验证。同时，明确硅酸钠提高马铃薯黑痣病抗性的应用方法。为马铃薯黑痣病提供环保、高效、安全、增产的新型防治措施，并深刻揭示其理论依据，具有理论和实践双重价值。

中文关键词： 马铃薯；硅酸钠；立枯丝核菌；抗病相关基因；功能分析

英文摘要： Stem canker and black scurf by Rhizoctonia solani is one of the most severe disease affecting potato production worldwide. It is serious in the growing areas of potato in Inner Mongolia.Farmers are still lack of effective measures to suppress the disease. Resistance of plant was enhanced by silicon and silicate has been confirmed in many diseases.In our previous research in field and laboratory, the resistance of underground stem and tuber of potato to Rhizoctonia solani were enhanced by sodium silicate,the yield and quality of potato were also increased. In this project, molecular mechanisms of resistance enhanced by sodium silicate will be researched mainly. The new transcriptome Illumina sequencing will be used and bioinformatics will be analysised to screen resistance-related genes. Real-Time PCR will be used to understand the spatial and temporal expression and function prediction of resistance-related genes. The whole length cDNA of the resistance-related genes will be cloned and the expression product will be analysised to determine the functional genes by RACE technique.Then the functions of these genes will be tested and validated in transformed potato. The same time, the using methods of sodium silicate will be determined. This research can find a new idea for controlling stem canker and black scurf of potato, and provide the theoretical basis for this new control measures that can protect environment, improve efficiency, increase production and is safe to human bings.

英文关键词： Potato;Sodium silicate;Rhizoctonia solani;Resistance-related genes;Functional analysis