SERS光流控芯片用于病原菌分类计数的研究

项目名称： SERS光流控芯片用于病原菌分类计数的研究

项目编号： No.21305125

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 刘湘江

作者单位： 浙江大学

项目金额： 25万元

中文摘要： 近年来，国内外食品安全事件频发，开发低成本、高通量的病原菌快速检测已刻不容缓。表面增强拉曼散射（SERS）技术利用病原菌自身拉曼光谱指纹进行检测，无需使用抗体和核酸探针，免除生物分子标记步骤，具有高灵敏度、高选择性、低成本等优势，但存在信号重现性差、信号采集方式效率低等问题。本项目拟将光流控芯片作为病原菌SERS检测的自动分析平台，解决以上两大问题。首先，针对病原菌SERS信号稳定性差的问题，在系统研究病原菌SERS信号产生的规律和影响因素的基础上，建立利用光流控芯片获得稳定病原菌SERS信号的理论和方法；其次，结合基于微芯片Coulter粒子计数器检测原理和相关化学计量学算法，建立可对病原菌SERS信号自动采集和分析的芯片系统，最终实现建立快速、低成本、高通量的病原菌分类和计数方法，满足有效预防与控制食源性疾病的需要。

中文关键词： 食品安全；表面增强拉曼光谱；病原菌检测；光流控；芯片实验室

英文摘要： Detection of pathogenic bacteria is hampered by the time-consuming enrichment and plating procedures of the traditional readout methods. Whilst the risk of microbial contamination in foods is rising, the need for a fast and accurate detection principle is increasing. Surface-enhanced Raman scattering (SERS) proves to be a powerful analytical technique for rapid identification of pathogenic bacteria Based on their own unique Raman spectral signature. However, the poor reproducibility of SERS detection and the lack reliable automatic readout methods limit its application in this aspect. Optofluidics devices, the combination of optical methods and microfluidic environments, are gaining popularity recently due to the flexibility and ease of optical detection. It has been proved that reliable SERS signal can be reproducibly generated in the microchannel of optofluidics chip. Hence, in this project, we proposes to develop SERS detection method of pathogenic bacteria on an optofluidics platform, since the detection can be both miniaturized and automated, making them faster and more economical. The proposed technique is of great importance in public health laboratories worldwide for rapid identifying pathogens responsible for outbreaks of foodborne illness.

英文关键词： Food safety；Surface-enhanced Raman scattering；Pathogen detection；Optofluidics；Lab on a chip