苯丙噻重氮（ASM）在马铃薯块茎快速愈伤中的作用机理研究

项目名称： 苯丙噻重氮（ASM）在马铃薯块茎快速愈伤中的作用机理研究

项目编号： No.31460412

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 王毅

作者单位： 甘肃农业大学

项目金额： 47万元

中文摘要： 马铃薯块茎损伤后会在短期内产生大量的活性氧（ROS），低浓度的H202作为信号分子可诱导一系列防御基因的表达。苯丙烷代谢为马铃薯块茎愈伤提供酚前体，而苯丙烷代谢的活化则需要能量的供应。我们前期研究发现ASM处理可以明显缩短马铃薯块茎的愈伤时间，但是，ASM处理后块茎体内的活性氧、能量供应及苯丙烷代谢如何变化，三者之间存在何种关系尚缺乏系统而又深入的研究。本项目拟以马铃薯块茎为试材，综合生理生化、细胞生物学和分子生物学等多学科方法，利用透射电子显微镜（TEM）、落视荧光显微镜、HPLC、蛋白组学分析及Western blot等技术手段，在亚细胞、生化和分子水平上解析ASM在促进马铃薯块茎快速愈伤过程中ROS的产生与清除，能量供应与苯丙烷代谢相关酶及产物的变化规律，以阐明ROS与能量、苯丙烷代谢之间的关系，为揭示ASM促进马铃薯块茎快速愈伤的作用机理提供理论依据。

中文关键词： ASM；马铃薯；愈伤；活性氧；苯丙烷代谢

英文摘要： In response to wounding, potato tubers generate a large number of reactive oxygen species (ROS). H2O2 at low concentration serves as a signaling molecule for up-regulating a series of defense-related genes. Phenylpropanoid metabolism supply phenolic precursors for wound healing of potato tuber, and energy is required for activation of phenylpropanoid metabolism. Our previous results showed that acibenzolar-S-methyl (ASM) treatment significantly shorted the duration for wound healing in tubers. However, the changes of ROS, energy supply and phenylpropanoid metabolism treated with ASM remain unknown, and the relationship among them need to be researched in systemic and in-depth. In this proposal, potato tubers are chosen as materials, and multidisciplinary approaches involving the physiological and biochemistry, cell biology and molecular biology, with the aid of modern analyzed methods such as transmission electron microscopy (TEM), epi?uorescence microscopy, high performance liquid chromatography (HPLC), proteomics analysis, and Western blot, the research is conducted to analysize the changes of activities of enzymes and metabolites related to ROS generation and scavenging, energy supply and phenylpropanoid metabolism in tubers treated with ASM at the subcellular, biochemistry, and molecular level. The results will elucidate complex relationships of ROS, energy and phenylpropanoid metabolism during wound healing, and also provide theoretical basis for further understanding mechanism of wound healing in potato tubers promoted by ASM.

英文关键词： ASM;Potato;Wound healing;Reactive oxygen species;Phenylpropanoid metabolism