RGS蛋白调控寡孢节丛孢菌丝分化及致病性的分子机理

项目名称： RGS蛋白调控寡孢节丛孢菌丝分化及致病性的分子机理

项目编号： No.31272093

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 杨金奎

作者单位： 云南大学

项目金额： 80万元

中文摘要： 全球每年由于植物寄生线虫引起巨大的农作物经济损失，食线虫真菌是自然界中线虫种群控制的重要因子，也是动植物线虫病害生物防治的重要研究材料。寡孢节丛孢是食线虫真菌的代表菌株，能够产生捕食器官捕捉线虫。我们的前期研究发现，寡孢节丛孢的基因组中存在7个G蛋白信号调节（RGS）蛋白，敲除AoRGS1的突变菌株不能产生捕食器官，对线虫的捕食能力急剧下降，说明RGS蛋白与捕食器官形成和致病性密切相关。为诠释RGS蛋白在寡孢节丛孢中的生物学功能和相关的调控机制，本项目拟采用Real time PCR方法分析RGS蛋白在寡孢节丛孢不同发育阶段的表达模式；通过同源重组技术敲除RGS蛋白的编码基因，阐明RGS蛋白的生物学功能；通过转录组技术比较突变菌株和野生菌株的基因表达差异，鉴定RGS蛋白调控寡孢节丛孢菌丝分化和致病性的相关基因，为揭示RGS蛋白调控寡孢节丛孢捕食器官形成和真菌生活模式转变的分子机制奠定基础。

中文关键词： 寡孢节丛孢；RGS蛋白；突变株；转录组；基因功能

英文摘要： Plant-parasitic nematodes cause severe damages to world agriculture every year, nematophagous fungi play important roles in maintaining nematode population density in natural environments, and they are important materials for biological control of animal and plant disease caused by nematodes. Arthrobotrys oligospora is one of the best-studied nematode-trapping fungi, which is capable of developing specific trapping devices (three-dimensional networks) to trap nematodes. In our previous study, seven regulators of G protein signaling (RGS) were predicted in the A. oligospora genome, the △AoRGS1 mutant could not produce the trapping devices and the nematicidal activity was declined dramatically, which suggested that RGS proteins were closely related to the trap formation and the pathogenicity of A. oligospora. In order to elucidate the biological function and regulation mechanism of RGS proteins in A. oligospora, we plan to study the expression pattern of RGS proteins in different growth stages of A. oligospora using real time PCR method; to reveal the biological roles of RGS proteins in A. oligospora by deleting the encoding gene of RGS by homologous recombination technology; to identify the genes involved in the regulation of hyphal differentiation and the pathogenicity of A. oligospora by RGS proteins using tran

英文关键词： Arthrobotrys oligospora；RGS proteins；mutants；transcriptome；gene function