魔芋葡甘聚糖经肠道菌发酵产生氢气及短链脂肪酸的构效关系

项目名称： 魔芋葡甘聚糖经肠道菌发酵产生氢气及短链脂肪酸的构效关系

项目编号： No.31301451

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 王凌

作者单位： 华中农业大学

项目金额： 25万元

中文摘要： 氢气的选择性抗氧化作用及对其他生理功能的影响近年获得了突破性进展，膳食纤维摄入并经由肠道菌群发酵产氢是内源性氢气的主要来源，但因在体实验中氢气产生、消除及作用途径复杂而鲜见研究；短链脂肪酸是同时产生的另一类重要产物，但其与氢气共存的生理活性研究尚处空白。本课题的目的是从氢气与短链脂肪酸组合物和选择性抗氧化的新视角，探明不同结构魔芋葡甘聚糖(KGM)通过肠道菌发酵后上述两类产物组成及其活性的差异。课题首先制备并表征不同结构KGM试样；通过体外厌氧发酵，检测它们对人粪便浆水体系中发酵产生氢气和短链脂肪酸的动态影响，比较各组发酵上清液对oOH和ONOO-等自由基的清除能力，观察其是否具有选择性抗氧化作用，并评价对Caco-2细胞毒性和基因毒性，以揭示KGM通过发酵产物影响抗氧化活性的构效关系；通过人工组配氢气和短链脂肪酸混合液，探讨其组成对上述体外活性的影响，为进一步改造KGM结构提供目标参考。

中文关键词： 魔芋葡甘聚糖；短链脂肪酸；肠道菌群；构效关系；膳食纤维

英文摘要： It is obtained breakthrough in research on selective oxidation of hydrogen and its influence on other physiology function in recent years. Endogenous hydrogen mainly derives from dietary fiber intake and being fermented by intestinal flora. However, this is far from adequate research that the path of hydrogen's production and elimination is complicated in vivo. Short chain fatty acids are another set of important products of the intestinal microflora fermentation. But the physiological activity of the coexistence of the two fermentation products were not investigated. The target of this study is to explore action mechanism of dietary fiber impacting human health and provide foundation of KGM based food ingredient development and science evaluation through new angle of hydrogen and short chain fatty acid generation and selective oxidation, using intestinal microorganism as medium. Products of different molecular weight and deacetylation degree are to be prepared and characterized. In vitro anaerobic fermentation system, the dynamic effect of former specimen on bacteria community structures and metabolic products (hydrogen，short chain fatty acid etc.) is to be compared; scavenging capacity of fermentation liquid to oOH and ONOO-, as well as its capacity of selective oxidation and its cytotoxicity and genotoxicity

英文关键词： Kanjac glucomannan；Short-chain fatty acids；Intestinal microflora；Structure-activity relationship；Dietary fiber