项目名称: 热激蛋白CPN10在组蛋白转录调控中的作用和机制研究
项目编号: No.31201017
项目类型: 青年科学基金项目
立项/批准年度: 2013
项目学科: 遗传学与生物信息学、细胞生物学
项目作者: 郑莉灵
作者单位: 浙江大学
项目金额: 23万元
中文摘要: 组蛋白是遗传物质?染色质的重要组成部分,其表达失调将会阻滞细胞周期进程。NPAT是调控组蛋白转录的重要因子,因此进一步识别其相互作用蛋白并研究其作用机制对于深入探索组蛋白转录的调控机制意义重大。本项目预实验首次发现了热激蛋白CPN10为NPAT的新相互作用蛋白,二者共同定位细胞核内。当内源性的CPN10 表达受到抑制时,组蛋白的转录水平也随之降低,证明CPN10是调控组蛋白转录的信号传导途径中另一重要蛋白。CPN10与NPAT之间的分子作用机理尚不明朗,而且与NPAT的上游基因CyclinE的关系以及对NPAT下游Oct-1-OCA-S的影响也有待进一步的深入探索。本研究拟通过各种生化手段结合显微荧光摄影技术,全面探索CPN10对NPAT及其上下游信号传导系统的作用,从而阐明CPN10在组蛋白转录调控中的分子机理。
中文关键词: 热激蛋白 Cpn10;组蛋白转录;NPAT 蛋白;histone locus body;细胞周期
英文摘要: Histone is an essential component of the heritage material-chromatin. Improper expression of histone will lead to cell cycle arrest. NPAT is a critical protein in the histone transcription signal pathway. Therefore, identification of novel NPAT binding partners will shed the light on the understanding of histone gene transcription mechanism. Our preliminary data showed that the heat shock protein CPN10 is a novel binding partner of NPAT which co-localizes with NPAT in the nuclear. Furthermore, knockdown of CPN10 dramatically reduces the histone mRNA level. All these data demonstrate the critical roles of CPN10 in histone gene transcription. However, the underlying molecular mechanism is elusive. In addition, the impact of CPN10 towards both NPAT and its upstream protein CyclinE is unknown. We will utilize bio-chemical techniques and confocal imaging to further study CPN10、NPAT and CyclinE-mediated histone gene transcription.
英文关键词: heat shock protein Cpn10;histone transcription;NPAT protein;histone locus body;cell cycle