二茂铁配合物@石墨烯核壳结构与癌细胞作用过程的QCM热动力学研究

项目名称： 二茂铁配合物@石墨烯核壳结构与癌细胞作用过程的QCM热动力学研究

项目编号： No.21473136

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 帅琪

作者单位： 西北农林科技大学

项目金额： 87万元

中文摘要： 石英晶体微天平(QCM)能够实时、快速、低样品消耗监测分子间相互作用过程并给出热动力学参数，作为一种新型生物传感器为药物筛选提供准确的热动力学信息。本项目拟设计合成系列新型二茂铁配合物@石墨烯核壳结构(@左侧为核，右侧为壳)，通过TEM、SEM及AFM对其尺寸和形貌进行表征。采用细胞毒性实验研究其对HeLa等癌细胞的抗癌活性，在得出活性规律的基础上，将癌细胞负载到芯片上，通过QCM实时监测四个温度下系列核壳结构与癌细胞作用过程，获得结合及解离速率常数，通过两者比值求出四个温度下作用过程的平衡常数，并由范特霍夫公式及作图法，进一步求出焓变、熵变及吉布斯自由能变。通过数据分析，探讨核壳结构的抗癌活性和热动力学数据的关系，对抗癌活性进行综合评估，进而对活性/非活性核壳结构药物筛选进行展望。

中文关键词： 石英晶体微天平；抗癌活性；热动力学；二茂铁配合物@石墨烯核壳结构

英文摘要： The Quartz Crystal Microbalance (QCM) technology allow measurements of thermodynamic parameters between moleculars such as carbohydrate-protein, protein-protein or guest-framework. This method is real-time, fast and consumes small amount of samples, which can provide useful thermodynamic information for selecting drug. Our project concerns with the ferrocene complex@graphene core-shell structures: Function-oriented synthesis and thermal dynamics study.The size and morphology of core-shell strcture can be characterized by TEM, SEM and AFM. The anticancer activity for cancer cells such as HeLa can be obtained by cytotoxicity test. The thermodynamic parameters for the interactions between core-shell structures and cancer cell can be monitored by QCM in the way of targeted modification and loading cell on a chip. Data (the association and dissociation rate constants, the equilibrium constant) will be obtained by full kinetic analysis at four different temperatures and final parameters (changes in enthalpy , entropy and Gibb's free energy) will be calculated using a Van't Hoff plot. From the data we obtained, we can discuss the relationships between the anticancer activity and the thermodynamic data, then give a prospect for the drug selection .

英文关键词： QCM;anticancer activity;thermodynamics;ferrocene complex@graphene core-shell structures