高原训练中运动员骨骼肌萎缩及分子网络调控机制研究

项目名称： 高原训练中运动员骨骼肌萎缩及分子网络调控机制研究

项目编号： No.31470059

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 胡扬

作者单位： 北京体育大学

项目金额： 30万元

中文摘要： 资料表明，高原训练的成功率只有40%，原因之一与高原训练中运动员力量丢失有关。理论上认为，高原训练中运动员力量丢失与高原低氧诱导的骨骼肌萎缩有关，但至今没有人体实验予以证实，更不了解其发生机制。高原训练中不仅存在低氧诱导骨骼肌蛋白质分解加强、合成减弱，进而导致骨骼肌萎缩的作用，也存在运动对骨骼肌合成的促进作用，其调控机制远比单纯的高原暴露复杂，有必要以分子网络为突破口，研究其调控规律与作用。本项目拟观察海拔3700m高原抗阻训练对运动员骨骼肌形态及收缩力量的影响，用肌肉活检技术提取运动员骨骼肌样本进行全基因组mRNA表达谱扫描，用生物信息学手段筛选分子调控的关键节点，并用分子生物学实验方法予以证实，以阐明高原训练中运动员骨骼肌萎缩的分子机制。

中文关键词： 高原；抗阻练习；骨骼肌萎缩；分子调控网络；

英文摘要： It was shown that only 40% of altitude training improved the athletic performance. The decrease of strength in athletes occurred in altitude acclimatization was believed as one of the most important factors,which caused the failure of the altitude training. Theoretically, the strength decrease is related to the hypoxia induced muscle atrophy during altitude exposure. However, no direct research focused on this phenomenon has been performed on human body and the underling mechanisms are still unknown. During the altitude training, hypoxia induces the muscular protein degradation and the synthesis suppression; on the other side, exercise facilitates the muscular protein synthesis. Therefore, the molecular regulation behind the interaction of the hypoxia and exercise stimuli must be more complicated than any one of the factors acting alone during altitude training. It is necessary to map the molecular regulation network to investigate the principles and mechanisms of the skeletal muscle atrophy during altitude training. In the project, we will investigate the effects of 2300m altitude training on the strength and morphologic parameters of skeletal muscle in athletes, test the human whole genome mRNA and miRNA expression with the muscle biopsy sampling from vastus lateralis, construct the regulation network of mRNA

英文关键词： altitude；resistance exercise；skeletal muscle atrophy；biomolecular regulatory networks；