硫化氢信号分子比例计量荧光探针的构筑与造影应用

项目名称： 硫化氢信号分子比例计量荧光探针的构筑与造影应用

项目编号： No.21271100

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 何卫江

作者单位： 南京大学

项目金额： 85万元

中文摘要： 硫化氢是继NO和CO之后的第三个气体信号分子，参与神经信号传递、信号转导及心血管功能调节等过程,而这些热门研究工作需要硫化氢的实时原位定量信息。由于荧光的环境依赖性、探针浓度未知和自发荧光干扰等因素，利用现有的增强型硫化氢荧光探针造影无法获得细胞及活体中硫化氢的定量信息。本项目提出通过构建具有硫化氢诱导发射或激发谱带移动的比例计量型荧光探针，利用其双带比例的内校正效应实现硫化氢在细胞或活体水平的实时比例成像，获得硫化氢的实时定量信息。项目提出构建分子内电荷转移(ICT)或荧光共振能量转移(FRET)机制的探针分子，通过硫化氢触发探针中敏感基团的反应诱导分子内ICT和FRET 效应的改变实现对硫化氢的比例响应。构建的近红外比例探针则可望用于裸鼠活体的硫化氢比例成像。项目的实施有望提供硫化氢定量跟踪造影的工具，不仅有利于促进硫化氢生物无机化学的发展，而且可推动对相关疾病诊疗技术的进步。

中文关键词： 硫化氢；荧光；成像；小鼠；细胞

英文摘要： As the third gaseous transmitter after nitric oxide (NO) and carbon monoxide (CO),hydrogen sulphide has been proposed to be associated with neurotransmission,singal transduction and regulation of cardiovascular functions, and the related research to understand the biological roles of hydrogen sulphide are attracting considerable attention, which requires the in situ quantitive real-time information of hydrogen sulphide in complicated living systems.Due to the autofluorescence, unknown sensor concentration, and the environment-dependence of fluorescence, the current "turn-on" fluorescent sensors for hydrogen sulphide displaying the hydrogen sulphide-induced emssion enahncement are disable to offer the quantitive temporal/spatial information of hydrogen sulphide in live cells or organism. The ratiometric hydrogen sulphide sensors of hydrogen sulphide-induced emission or excitation shift was proposed to be developed in this proposal to realize the real-time ratiometric imaging of inctracellualr or in vivo hydrogen sulphide homeostasis and offer the quantitive hydrogen sulphide information, profiting from the internal caliberation effect via determining the dual emission ratio between two excitation or emission bands. With the normal ratiometric sensing mechanisms of internal charge transfer (ICT) and F?rster reson

英文关键词： hydrogen sulfide；fluorescence；imaging；mouse；cell