c-Src促进鼻咽癌转移的作用与临床意义的研究

项目名称： c-Src促进鼻咽癌转移的作用与临床意义的研究

项目编号： No.81201630

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 肿瘤学1

项目作者： 吕星

作者单位： 中山大学

项目金额： 23万元

中文摘要： 远处转移是鼻咽癌治疗失败的主要原因。我们通过高通量蛋白芯片分析不同预后的鼻咽癌患者血清蛋白谱，首次发现血清中c-Src浓度水平是鼻咽癌患者远处转移的预测指标；并且进一步的预实验也证实c-Src在鼻咽癌组织中的表达与患者临床分期呈正相关；在细胞实验中，我们发现c-Src可以促进鼻咽癌细胞的迁移和侵袭。因此我们提出科学假设：c-Src具有促进鼻咽癌转移的作用，并且其表达水平能够预测鼻咽癌患者的预后。本项目拟通过高/低转移鼻咽癌细胞株的体外研究、裸小鼠转移模型和临床标本检测，探讨c-Src与鼻咽癌患者的临床特征和预后的关系，研究c-Src对鼻咽癌细胞上皮－间叶表型转化过程的影响，证实c-Src具有促进鼻咽癌转移的作用。本项目的完成对预测鼻咽癌患者的预后具有重要意义，也将为防治鼻咽癌的远处转移提供新的靶点。

中文关键词： 鼻咽癌；转移；c-Src活化；靶向治疗；上皮-间叶转化

英文摘要： For nasopharyngeal carcinoma (NPC) patients, distant metastasis remains the major cause of treatment failure. Through analysing serum protein spectrum from different prognosis of NPC patients by high-throughput protein chip, we firstly found that serum c-Src concentration level was the independent predictor of poor DMFS of NPC patients, then the pre-test also confirmed that the expression of c-Src in tumor organization was related with patients clinical staging. In vitro, we found c-Src protein promote cell migration and invasion in NPC. So we make a scientific hypothesis: C-Src has the role of promoting metastasis of NPC and its expression levels can predict the prognosis of NPC patients. This project will explore, through high/low metastasis of NPC cell lines in vitro and metastasis model in nude mice and clinical specimens, that the relationships between c-Src and clinical characteristics/prognosis in NPC patients, explore the role of c-Src in NPC cells of epithelial to mesenchymal transition process, and to confirm that c-Src can promote the metastasis of NPC. Completion of this project will be to predict the prognosis of NPC patients, as well as provide new target of prevention and cure of NPC with distant metastasis.

英文关键词： nasopharyngeal carcinoma；metastasis；c-Src activation；target-therapy；epithelial-mesenchymal transition