基于连锁作图与关联分析发掘辣椒疫病抗性基因

项目名称： 基于连锁作图与关联分析发掘辣椒疫病抗性基因

项目编号： No.31460520

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 陈学军

作者单位： 江西省农业科学院

项目金额： 50万元

中文摘要： 抗病基因发掘是农作物抗性遗传改良的重要基础。辣椒疫病（Phytophthora blight）是由疫霉菌（Phytophthora capsici L.）侵染引起的一种毁灭性辣椒病害，其抗性遗传机理复杂。本研究以项目组基于'C. annuum V06C1720'（高感）× 'C. annuum V06C1825' （高抗）构建的RIL（F6，n=201）为连锁作图群体、辣椒初级核心种质（n=405）为关联分析群体，在系统开展疫病抗性鉴定和SSR标记全基因组扫描基础上，联合应用连锁作图和关联分析方法，全面剖析辣椒疫病抗性变异，发现并精细定位辣椒疫病抗性基因（QTLs），获得紧密连锁的SSR分子标记，挖掘具有潜在育种价值的优异等位变异及其载体材料。研究结果将为辣椒疫病抗性分子标记辅助选择育种、抗性基因克隆以及抗性遗传改良奠定坚实基础。

中文关键词： 辣椒疫病；抗性基因；连锁作图；关联分析；发掘

英文摘要： Identification on resistance genes is an important basis of crop resistance genetic improvement. Phytophthora blight in pepper is caused by Phytophthora capsici Leonian, causing severe damage to Capsicum crops, and the disease occurs widely in pepper cultivation areas in the world. The genetic mechanism of resistance of pepper to the disease is complex. In this study, we will conduct both linkage mapping and association analysis to overall dissect the variation of the resistant traits and identify the resistance genes (QTLs) to P. blight based on resistance identification and genotyping with genome-wide SSR markers. The linkage mapping was based on a RIL population (F6, n=201) developed from 'C. annuum V06C1720'(susceptible) × 'C. annuum V06C1825' (resistant) cross, and the association mapping was applied to 405 accessions of the primary core collection. SSR markers tightly linked to P. blight resistance gene will be screened and the excellent alleles having potential value in pepper breeding and their carrier materials will be identified from the research. The study will lay solid foundation for the molecular marker-assisted breeding, resistance genes cloning and resistance genetic improvement in pepper.

英文关键词： Phytophthora blight in pepper;resistance genes;linkage mapping;association analysis;Exploration