卵转铁蛋白增强巨噬细胞抗病毒天然免疫作用的分子机制

项目名称： 卵转铁蛋白增强巨噬细胞抗病毒天然免疫作用的分子机制

项目编号： No.31460432

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 徐明生

作者单位： 江西农业大学

项目金额： 52万元

中文摘要： 卵转铁蛋白（OVT）是禽蛋中主要活性蛋白之一，具有较好的抗病毒作用。我们前期研究表明OVT不影响水泡口炎病毒（VSV）与巨噬细胞的结合，但能显著地抑制VSV在小鼠腹腔巨噬细胞内的复制。OVT这种抗病毒作用是通过何种机制来实现的？目前尚未见相关报道。本研究拟采用体外细胞培养模型，利用实时荧光定量RT-PCR、ELISA和Western Blot 等技术分析OVT对VSV诱导巨噬细胞干扰素基因转录、干扰素分泌及相关蛋白的表达的影响；研究OVT对巨噬细胞经TLRs、TfR诱导的核转入因子活化的影响，及活化的转入因子与TLRs 或RIG-I介导的INF-I表达的相关性。从细胞和分子水平阐明OVT增强巨噬细胞抗病毒天然免疫作用的分子机制。本研究结果可为卵转铁蛋白作为食品功能因子在保健食品中的应用提供理论依据，对禽蛋的深度开发利用具有重要的影响。

中文关键词： 卵转铁蛋白；巨噬细胞；抗病毒；分子机制

英文摘要： Ovotransferrin(OVT) is one of the major bioactive compounds in egg, and has good antiviral activities. Our previous research work demonstrated that OVT could significantly inhibit the vesicular stomatitis virus (VSV) replication on mouse peritoneal macrophages, but could not affect the binding of the virus on macrophages. Is the antiviral function of OVT achieved by the way of stimulating gene expression and then elevating the antiviral cytokine levels, or via any other mechanisms? All these questions need to be addressed. In this study, qRT-PCR, ELISA, Western Blot, and other comtemperary and conventional methodologies will be used to determine the effects of OVT on the secretion of type I interferon and related proteins expressions of macrophages induced by VSV in vitro. Meanwhile, the effects of OVT on the nuclear transfer factor activation in macrophages induced by TLRs and TfR will be studied to analyze the correlationship between the signal pathway mediated by TLR or RIG-I and INF-1 expression, and thus to elucidate the molecular mechanisms of the OVT enhancing macrophages antiviral innate immunity at the cellular and molecular levels. The expected results will provide a theory basis for OVT being used as a functional factor in health food producion, which will have profound impacts and academic values for the further utilization of the OVT.

英文关键词： Ovotransferrin;Macrophages;Antiviral;Molecular Mechanism