基于多管离子选择性微电极的番茄营养水平快速检测方法

项目名称： 基于多管离子选择性微电极的番茄营养水平快速检测方法

项目编号： No.31201659

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 植物保护学、园艺学与植物营养学

项目作者： 左志宇

作者单位： 江苏大学

项目金额： 23万元

中文摘要： 以温室栽培的番茄为对象，从多管离子选择性微电极制备和信号解析两方面入手，研究多管离子选择性微电极的离子检测机理和基于离子浓度的番茄营养水平评价方法。通过对番茄叶片和维管组织的显微结构分析，采用有限元法建立力学模型，寻求最优的微电极形状和结构参数；筛选或研制合适的敏感膜，研究在微米级尺度下各敏感电极的电信号特征，构建微电极信号－离子浓度测量模型。研究番茄在不同氮、钾、镁、钙等营养水平下叶片和维管组织内硝酸根离子、铵离子、钾离子等作物营养离子浓度的变化规律，建立营养元素营养水平与离子浓度的关系模型；对这些关系模型进行解析，探索根据离子浓度信息反演营养水平的方法。分析微电极信号与植株体内离子浓度的变化规律，提取营养水平特征信息，采用主成分分析方法和模糊神经网络方法，建立营养信息检测模型。本项目突破了传统微电极无法同时、精确检测作物氮、钾、镁、钙营养水平的瓶颈。

中文关键词： 番茄；多管离子选择性微电极；营养水平；快速检测；模型

英文摘要： An evaluation method is investigated for greenhouse tomato nutrient level based on ion concentration using multi-barrel ion-selective microelectrodes. The study is started from multi-barrel ion selective microelectrode preparation and signal resolution. By analyzing the microstructure of tomato leaves and vascular tissue, the mechanical models are established using the finite element method, thus finding the optimal microelectrode shape and structural parameters. The suitable sensitive membranes are screened or developed. Studies are made for the electrical signal characteristics of sensitive electrode that its tip is less than 10 micron. Then the measurement models are constructed between microelectrode signal and ionic concentration. The variations are studied among nitrate, ammonium, potassium, calicium and magnesium ions concentration of tomato leaves and vascular tissue under different elements of nitrogen, potassium, magnesium, calcium nutrient levels, thus constructing the models between nutrient levels and ionic concentrations. The model is analyzed to explore the measurement of inversing nutrition levels according to the ionic concentration. The variation was analyzed between microelectrode signal and the ion concentration. The feature information is extracted for nutritional level using principal compo

英文关键词： tomato；multi-barrel ion-selective microelectrode；nutrient level；rapid detection；model