香蕉枯萎病菌响应韭菜胁迫的转录组分析及相关基因功能研究

项目名称： 香蕉枯萎病菌响应韭菜胁迫的转录组分析及相关基因功能研究

项目编号： No.31471864

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 黄永红

作者单位： 青岛农业大学

项目金额： 87万元

中文摘要： 香蕉枯萎病是毁灭性病害，目前尚无根除措施。我们研究发现，采用韭菜轮作/套种香蕉的种植模式能显著减少香蕉枯萎病的发生。目前该模式已成功应用于生产实践中，并初步取得良好的经济效益和社会效益。但韭菜的防病机理尚不明确。我们进一步发现韭菜提取物能显著抑制香蕉枯萎病菌4号生理小种（Foc4）菌丝的生长、破坏菌丝的结构，还能抑制孢子萌发、高效杀灭孢子。在国家自然基金（31272151）的资助下，我们从韭菜中分离鉴定到6种能高效抑制Foc4生长的活性成分。本研究中拟用分离到的活性物质对Foc4进行处理，利用转录组测序技术筛选Foc4中韭菜活性物质作用的靶标基因，并通过基因敲除技术对筛选到的靶标基因进行功能分析鉴定，以期从分子水平揭示韭菜抑制香蕉枯萎病发生的机制，为研发高效防控香蕉枯萎病的植物源药剂提供理论基础，也为通过分子生物学手段防控香蕉枯萎病提供宝贵的基因资源与理论支撑。

中文关键词： 香蕉枯萎病；韭菜；转录组测序；基因敲除；功能分析

英文摘要： Banana Fusarium wilt is regarded as one of the destructive plant diseases. In addition, there are still no methods to completely control thefetal disease. After several years of investigations, we found that adopting the Chinese leek-banana rotation/intercropping system could significantly decrease the incidence of Fusarium wilt in banana. Presently, the planting mode has already been applied in commercially practical production and has created primary social and economic benefits. However, the mechanism of Chinese leek on the control of Fusarium wilt in banana is still not clear. The results of our further study suggested that Chinese leek extract inhibited the growth of Fusariuim oxysporum f.sp. Cubense Race4(Foc4）hypha, destructed the hypha structure,suppressed germination of the spores,and also caused efficient death to the spores. Supported by the National Natural Science Foundation of China (Grant No. 31272151), we isolated and identified six antifungal components from Chinese leek that significantly inhibted the growth of Foc4.In the project, we will stress Foc4 by using the newly isolated antifungual components,tring to screen target genes in Foc4 respond to the antifungual components by using transcriptome sequencing,and to identify the function of the screened target genes by using gene knock-out technique.The project will reveal the molecular mechanism of the reduction of Fusarium wilt in banana caused by Chinese leek,provide theoretical base to the research and development of botanical reagents effectively controlling the Fusarium wilt and provide precious gene resource to the study on controlling the fetal disease by using molecular biotechnology means.

英文关键词： Fusarium oxysporum f. sp. cubense;Chinese leek;Transcriptome sequencing;Gene knock-out;Functional analysis