抵抗素在膀胱癌发生发展中的作用及机制研究

项目名称： 抵抗素在膀胱癌发生发展中的作用及机制研究

项目编号： No.81202023

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 肿瘤学2

项目作者： 张朋

作者单位： 四川大学

项目金额： 23万元

中文摘要： 膀胱癌是我国泌尿生殖系统最常见的肿瘤，发病率及死亡率均占泌尿生殖系统肿瘤首位，其较高复发率、进展率及远处转移率严重威胁患者生存率。近年来研究表明，脂肪细胞因子家族如瘦素、脂联素、抵抗素及内脏脂肪素等与肿瘤的发生发展关系密切。抵抗素（Resistin）是具有免疫调节功能的脂肪细胞因子，在肿瘤细胞凋亡调控中发挥着重要作用，可能是通过激活PI3K、MAPK或NF-κB通路而参与肿瘤的发生发展。我们的前期研究证明膀胱癌组织中Resistin表达明显增加，提示Resistin与膀胱癌的发生发展密切相关。课题组拟从DNA遗传变异、蛋白亚细胞定位、mRNA与蛋白表达水平等角度，深入研究Resistin与膀胱癌发生、发展、侵袭与转移的关系及分子机制。采用质粒转染及RNA干扰技术干预Resistin的表达，研究其对膀胱癌细胞凋亡或存活的影响及其信号通路，探索干预Resistin表达调控与膀胱癌发生发展的关系

中文关键词： 膀胱癌；抵抗素；发病风险；生存分析；分子机制

英文摘要： Bladder cancer is the most common cancer of genitourinary system in China, morbidity and mortality account for the first of genitourinary tumors. The survival rate of patients was affected by the high recurrence, progression and distant metastasis rates. Recently, studies have shown that the adipocytokine family such as leptin, adiponectin，resistin and visfatin associated with tumor genesis and development are closely related. Resistin is an adipocytokine with immunomodulatory function, which play an important role in the regulation of tumor cell apoptosis. Resistin is involved in tumor genesis and development possibly through the activation of PI3K, MAPK or NF-κB pathway. Our previous work have shown that bladder cancer tissue resistin expression was significantly increased, suggested that there is closely related about resistin and bladder cancer genesis. Our team intends to work at the DNA genetic variation, subcellular protein position, the mRNA and protein expression levels to reveal the relationship between resistin and bladder cancer. The expression of resistin is effected by plasmid transfection and RNA interference, research the cell survival rate and signal pathway. Explore the relationship of regulation resistin expression and bladder cancer genesis or development or metastasis.

英文关键词： bladder cancer；resistin；risk；survival；molecular mechanism