一氧化氮调控植物硒代谢机制研究

项目名称： 一氧化氮调控植物硒代谢机制研究

项目编号： No.31260057

项目类型： 地区科学基金项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 肖强

作者单位： 湖北民族学院

项目金额： 48万元

中文摘要： NO作为信号分子广泛参与了对植物生长发育和生理生化过程调节；Se是人体必需的微量元素，也是植物的有益元素。由于其分布在地球上极不均匀，获得富硒植物并对高硒污染环境进行有效生物修复具有重要理论和实践意义。本研究项目以不同内源NO水平模式植物拟南芥为材料；在研究方法上，运用了muXRF、XANES和HPLC-ICP-MS分析实验材料中硒累积、空间分布和化学种类，荧光探针、化学发光法测定NO，RT-PCR分析Se吸收、转运、代谢相关基因表达水平变化，生物素标记(biotin switch)方法研究拟南芥硒代谢相关蛋白S-亚硝酰化修饰；在研究路线上，采用抑制剂实验、NO供体与清除剂实验、突变体实验等相结合的办法，综合研究分析NO对植物Se吸收、代谢的调节机制。预期结果可深化人们对NO在植物中的作用模式认识并为通过生物工程手段改善植物Se富集和生物修复能力提供理论依据。

中文关键词： 亚硒酸钠；一氧化氮；硒吸收；硒代谢；基因表达

英文摘要： Accumulated evidence demonstrates that nitric oxide (NO) are signals to participate in multiple physiological processes, including systemic acquired resistance, the hypersensitive response, leaf senescence, programmed cell death, stomatal closure, root gravitropism, cell wall development, flowering repression, and development. Selenium (Se) is an essential micronutrient for many organisms, including animals and humans. It is also a beneficial nutrient for many plants. Soil Se concentration and bioavailability vary with parent material and environmental conditions, and the distribution of Se in soils is usually heterogeneous and site-specific. As plants are the main source of dietary Se, plant Se metabolism is therefore important for Se nutrition of humans and other animals. However, the concentration of Se in plant foods varies between areas, and too much Se can lead to toxicity. It is imporant in the current understanding of Se in higher plants,as well as advances in the genetic engineering of Se metabolism, particularly for biofortification and phytoremediation of Se-contaminated environments. In this work , speciation of selenium in Arabidopsis thaliana which has different endogenous NO level is performed via size-exclusion chromatography and ion-pairing reversed phase chromatography using inductively coupled

英文关键词： Selenite；nitric oxide；Se uptake；Se metabolism；gene expression