角果碱蓬适应碱性盐土壤的分子基础初探

项目名称： 角果碱蓬适应碱性盐土壤的分子基础初探

项目编号： No.31300305

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 庞秋颖

作者单位： 东北林业大学

项目金额： 22万元

中文摘要： 碱蓬属植物为世界广布、耐盐能力极强的真盐生植物。我们通过野外观察并分析碱蓬属植物在盐碱土壤上的分布特点发现，角果碱蓬更适应于含碱性盐（碳酸盐，pH较高）的土壤，极少见于松嫩盐碱土壤以外的其他地域。本项目应用蛋白质组学和代谢组学技术比较角果碱蓬的生理代谢对碱性盐（Na2CO3）、中性盐（NaCl）胁迫的响应与适应过程，并结合生物信息学手段初步解析角果碱蓬适应碱性盐胁迫的分子基础。这些系统生物学的研究方法可以弥补以往研究中仅"局部"观察少数生物学指标或生理学现象的不足，同时有利于甄别角果碱蓬与碱性盐、中性盐相关的典型代谢特征和特异代谢途径，提高了深入认知角果碱蓬适应碱性盐土壤机制的可能性，有益于深入理解盐生植物对盐碱环境的适应机理，也是深入挖掘碱性盐土壤适宜植物资源的重要基础工作。

中文关键词： 角果碱蓬；碱性盐；蛋白质组学；代谢组学；松嫩盐碱草地

英文摘要： Plants of Suaeda are the typical halophyte with a worldwide distribution and adaptive to the high salinity. Based on the analysis of the field distribution of Suaeda spieces on Songnen alkali grassland, we found that S. corniculata is principally on Songnen saline-alkaline grassland in distribution, which exhibits strong tolerance to alkaline salts with high pH (NaHCO3 and Na2CO3). Proteomics and metabolomics is applied to investigate the physiological responses and the adaptive changes of S. corniculata exposed to high concentrations of the typical neutral salts and alkaline salts in this project. Bioinformatics analysis is conducted based on the analyzing the molecular foundations of alkaline salt adaptation in halophyte S. corniculata. Systems biology focuses on complex interactions in biological systems which is complementary to the investigations of the traditional physiology analysis that limited in the number of biological indicators or phenotypes. Systems biology research would help to recognize typical metabolites profiling and unique metabolic pathway in the plants exposed to different types of salts. To investigate the differences in the systematic responses to neutral salt and alkaline salt stress in S. corniculata would help us to understand salinity tolerance in halophytes and would also aid in the

英文关键词： Suaeda corniculata；alkali salt；proteomics；metabolomics；Songnen alkali grassland