基于纳米电极表面离子开关与电化学催化协同效应的miRNA检测方法研究

项目名称： 基于纳米电极表面离子开关与电化学催化协同效应的miRNA检测方法研究

项目编号： No.21205079

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 分析化学

项目作者： 刘刚

作者单位： 上海市计量测试技术研究院

项目金额： 23万元

中文摘要： 微小RNA（miRNA）具有调控基因表达的作用，是潜在肿瘤分子靶标，但其分子短、丰度低、序列高度相似，给检测方法提出挑战。本项目提出基于纳米针尖电极表面离子开关与电化学催化协同效应，构建新型miRNA检测方法。在纳米金电极表面组装肽核酸（PNA）探针，特异性识别目标miRNA；探针与miRNA杂交后从柔性松散单链变成刚性直立双链，在电极表面形成铁氰离子扩散开关效应；且电极表面的miRNA可增加六氨合钌离子吸附量，提高催化电化学信号，实现目标miRNA检测。本项目将"离子开关"效应与电化学催化结合，提高检测灵敏度；采用纳米尺度电极，降低了样品需求量；改进表面探针，降低非特异吸附，实现高灵敏度、高特异性、无标记miRNA检测。我们将结合先进表征技术（STM，TEM，XPS等），综合考察各反应条件，阐述机理，形成其他纳米生物传感研究可借鉴的理论模型，为临床诊断应用奠定基础。

中文关键词： 纳米电极；微小RNA；电化学生物传感器；电化学催化；

英文摘要： MicroRNAs (miRNA) are short (～22 nucleotides) non-coding RNA molecules that regulate gene expression. Since their dysregulation leads to development of cancers, miRNAs have recently been proposed as being useful in diagnostics as biomarkers. Unfortunately,their characteristics like low abundance ,high degree of sequence similarity and extremely small size of mature miRNAs make it challenging to analysis miRNA with high sensitivity and specificity. Recently, nanoelectrochemical miRNA biosensors attract a lot of research interest for their advantages of sensitivity, small amount of starting sample and label-free analysis. In this project, we firstly assemble the PNA detection probes onto the surface of a nano-tip electrode. With the hybridization of target miRNAs, the probe will turn their configuration from free soft single-strand to rigid double-strand, which will construct a special "ion-gating" effect of [Fe(CN)6]3- on the surface of the nanotip electrode. In the meantime, the adsorption of [Ru(NH3)6]3+ will be increased for the increased negative charge on the electrode surface. As a result, the electrocatalysis signal of RuIII/FeIII will be amplified. This method is constructed on a nano-sized electrode, which need less amount of starting materials. We combined the "ion gating" effect with the electrocatalys

英文关键词： nanoelectrode；miRNA(microRNA)；electrochemical；electrocatalysis；