mPFC神经环路中突触结构重塑与慢性应激大鼠抑郁样行为的关系研究

项目名称： mPFC神经环路中突触结构重塑与慢性应激大鼠抑郁样行为的关系研究

项目编号： No.81501173

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 李晓莉

作者单位： 东南大学

项目金额： 17.5万元

中文摘要： 近年来，抑郁症 “突触发生假说”备受关注，而内侧前额叶（medial prefrontal cortex，mPFC）神经环路功能失调被认为与心境障碍的发生具有密切作用。本项目拟通过建立慢性不可预见性应激模型，运用电镜、高尔基染色、western blot、Real-time PCR和激光共聚焦等技术，检测慢性应激和艾司西酞普兰抗抑郁治疗后大鼠mPFC神经环路中重要情绪相关脑区-mPFC的前边缘皮层区（prelimbic cortex，PrL）、扣带回1区（area 1 of cingulate cortex，Cg1）、海马CA1、CA3、DG亚区和杏仁核BLA亚区中突触超微结构重塑情况及其可能的分子机制，分析突触超微结构重塑与大鼠抑郁样行为发生的关系，从而探讨抑郁症的可能病理生理机制，寻找抗抑郁治疗的新靶点提供新的思路和切入点，最终为为抑郁症的“突触发生假说”提供更多的实验依据和支持。

中文关键词： 突触可塑性；抑郁样行为；抗抑郁治疗；情感神经环路；抑郁症

英文摘要： The “Synaptogenesis Hypothesis” of depression attracts more and more attention recently. The dysfunction of medial prefrontal cortex (mPFC) neural circuit is considered as playing very important roles in the mood disorder. In the present study, we planned to examine the changes of synaptic remodeling and its possible molecular mechanism in several brain regions of mPFC neural circuit in rats after chronic stress and/or escitalopram treatment, including the prelimbic cortex and area 1 of cingulate cortex in mPFC, hippocampal CA1, CA3 and DG, as well as the basolateral amygdaloid nucleus. Then, the relationship would be analyzed between ultrastructural remodeling of synapses in different regions and depressive-like behaviors of rats. Therefore, the study was designed to reveal the possible physiopathologic mechanisms of depression, and provide more information to support the “Synaptogenesis Hypothesis” of depression.

英文关键词： synaptic plasticity;depressive-like behaviors;anti-depressant treatment;mood neural circuits;depression