恶性肿瘤与冠心病血瘀证TGF-β/Treg介导免疫失衡的差异性研究

项目名称： 恶性肿瘤与冠心病血瘀证TGF-β/Treg介导免疫失衡的差异性研究

项目编号： No.81202838

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 许云

作者单位： 中国中医科学院西苑医院

项目金额： 23万元

中文摘要： 血瘀是冠心病与恶性肿瘤共同的主要病理因素，活血化瘀已成为冠心病治疗的主流，但在恶性肿瘤治疗中却备受质疑。免疫失衡是疾病发生发展的根本内在因素。我们既往的临床和实验研究表明，无论是冠心病还是恶性肿瘤，均存在一定程度的免疫失衡。Treg细胞的数量和功能状态对机体免疫稳态的维持有重要意义，TGF-β1是维持Treg细胞数量和功能的重要因子,以他们介导的免疫失衡在冠心病与恶性肿瘤患者体内却呈现不同甚至是相反的状态。因此，我们设计了TGF-β/Treg介导的血清T细胞亚群、细胞因子及相关蛋白表达失衡为切入点，研究晚期恶性肿瘤和冠心病血瘀证的免疫失衡状态的差异性，以及其不同的免疫失衡水平与中医兼证、不同西医分型、凝血指标、疾病预后等因素的相关性，为不同疾病血瘀证本质的深化研究提供依据，为恶性肿瘤血瘀证的微观辨证、筛选有效的活血化瘀中药及作用靶点、为辨病与辨证相结合在临床中的应用提供分子基础。

中文关键词： 恶性肿瘤；不稳定型心绞痛；血瘀证；Treg；TGF-β

英文摘要： Blood stasis is the common main pathological factors of the coronary heart disease and malignant tumor. Promoting blood circulation and removing blood stasis has become the mainstream treatment for cornoary heart disease, but still is questioned for malignant tumor. Immune imbalance is the fundamental internal factors of the development in disease. Our previous clinical and experimental studies have shown, either coronary heart disease or malignant tumors, have a certain degree of immune imbalance. It is important of the number and function of Treg cells on immune homeostasis maintenance, and TGF-β1 is the important factor for maintenance of Treg cell number and function. There is different or even opposite state of them mediated immune imbalance in the patients with coronary heart disease patients with malignant tumor. Therefore, regarding TGF-β/Treg-T as the axis of the serum T cell subsets, cytokines and related protein expression imbalance as the breakthrough point, we design this study on the difference of immunue imbalance in blood stasis syndrome between advanced malignant tumor and coronary heart disease, as well as the correlation of different immune imbalance level and TCM syndrome types, and different Western medicine type, coagulation parameters, and disease prognosis, to provied a basis for the deep

英文关键词： malignant tumor；unstable angina pectoris；blood stasis syndrome；Treg；TGF-β