茉莉酸调控水稻花器官发育分子调控网络的研究

项目名称： 茉莉酸调控水稻花器官发育分子调控网络的研究

项目编号： No.31470397

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 袁政

作者单位： 上海交通大学

项目金额： 85万元

中文摘要： 禾本科包含水稻、小麦等重要的农作物，该家族植物具有特异的花器官形态特征，因其在生产中的重要性和形态发育上的多样性，其器官起始、发育和形态建成的遗传和分子调控机制的研究一直以来是植物学和育种学家的热点研究领域之一。水稻花器官具有单子叶植物特有的形态结构，其发育正常与否直接影响到水稻后代的繁殖和种子的产量。本项目将在前期研究工作的基础上，以JA生物合成代谢和信号传递异常突变体eg1-3和eg2-1D为研究材料，筛选eg1-3的回复子并对回复调控基因的功能进行分析；利用酵母双杂交技术筛选EG2/OsJAZ1基因的互作蛋白；利用RNAseq技术筛选OsJAZ1和OsMYC2的下游基因；利用CRISP等基因定点突变技术获得osmyc2等JA响应下游基因突变体，以期系统地开展JA调控水稻生殖发育时期花器官原基起始和后期形态建成的分子调控网络，为深入研究JA信号调控网络的进化机制奠定基础。

中文关键词： 水稻；花；茉莉酸；形态建成；调控网络

英文摘要： Poaceae (also called grasses or Gramineae) is one of the largest families of angiosperms. It contains many agriculturally important crops, such as domesticated rice (Oryza sativa), barley (Hordeum vulgare), wheat (Triticum aestivum), maize (Zea mays), and sorghum (Sorghum bicolor). All grasses have a characteristic and complex inflorescence composed of one, a few, or many spikelets with greatly diversified organizations. Research on grass inflorescence diversity, development, and genes network associated with morphogenesis is always interested to biologist and breeders because of their significantly contribution to our understanding of the development of grass inflorescences and spikelets, which will greatly facilitate crop breeding programs for grain yield increase. Rice contains specialized spikelets, structural units of inflorescences that show great morphological diversity even within the grass family. In this research, we will further focus on uncovering the molecular mechanism of JA in controlling rice spikelet development by screening enhancer or attenuator of eg1-3 and interacting proteins with OsJAZ1, isolating downstream genes of OsJAZ1 and OsMYC2, and we will do site-directed mutagenesis such as TALEN and CRISP to mutated genes selected from Y2H and RNAseq assay, and to perform gene functional study by combining genetic, bioinformatics and biochemical methods. These research will not only help us get a clearer glimpse about the underlying regulatory network of JA signaling in controlling rice flower development, but also provide a chance to understand the evolutionary mechanism of JA signaling in regulating plant development.

英文关键词： rice;flower;jasmonic acid;morphogenesis;regulatory network