基于集成化双酶-量子点纳米探针的化学发光生物分析新方法

项目名称： 基于集成化双酶-量子点纳米探针的化学发光生物分析新方法

项目编号： No.21305009

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 许淑霞

作者单位： 成都理工大学

项目金额： 25万元

中文摘要： 化学发光共振能量转移（CRET）在生命分析中已显示了其特有的优势如无需光源、仪器简单、反应设计多样化等，将其与独特光学性能的量子点结合，研究的化学发光共振能量转移量子点（CRET-QDs）探针在生命分析中具有良好的应用前景及实用价值。本项目旨在将双酶体系即辣根过氧化物酶（HRP）和氧化酶引入到量子点中，利用HRP标记抗体、氧化酶标记抗原通过抗原-抗体特异性识别作用构建新型集成化的双酶-量子点纳米探针及相应的CRET新体系，并在此基础上，研究直接多组分分析方法和免疫多组分分析方法以用于氧化酶底物、心肌疾病相关的代谢物及肿瘤标志物等的快速检测。该集成化的新型双酶-量子点纳米探针可实现在量子点表面微区内偶联的酶促反应，不仅具有高的发光反应效率和共振能量转移效率，而且具有无需加入氧化剂、极低的化学发光背景、低试剂消耗等优点，故可有效地促进量子点探针在生命分析中的应用。

中文关键词： 双酶；量子点；化学发光；共振能量转移；

英文摘要： The technique based on chemiluminesecence ressonance energy tansfer (CRET) has shown its unique advantages in the bionalysis such as no light source, simplicity of instrument, variety of chemiluminescence reaction system, etc. Combining CRET with quantum dots (QDs), CRET-QDs probes have been deveolped with the potential of good appllication in bionalysis. In this project, the bienyme system, i.e. horseradish peroxidase and oxidase,are going to be introduced into QDs probes for fabrication of a novel integrated bienzyme-QDs nanoprobe and corresponding CRET system. The construction of integrated probe is based on the recognition between antibody and antigen which are labled with horseradish peroxidase and oxidase, respectively. Utilizing the new probe and CRET system, multiplexed analytical methods will be established for direct determination of oxidase substrates and immunoassay of the tumor markers and the metabolisms related to cardiomyopathy. In the fabricated bienzyme-QDs nanoprobe, the coupled enzyme-catalyzed reactions will be conducted at the microarea of QDs surface, thus accopanying high reaction and energy transfer efficiency. In addition,it provides the advantages of elimination of the oxidant,ultralow chemiluminescence background and low reagent consumption. The accomplishment of this project will f

英文关键词： Bienzyme；Quantum dots；Chemiluminescence；Resonance energy transfer；