食道上皮基膜的结构分析及组织工程化食道上皮的构建

项目名称： 食道上皮基膜的结构分析及组织工程化食道上皮的构建

项目编号： No.30870645

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 金属学与金属工艺

项目作者： 竺亚斌

作者单位： 宁波大学

项目金额： 28万元

中文摘要： 基膜是位于上皮细胞基底面与结缔组织之间的多孔纳米纤维膜，具有支持和连接作用。它也是一种储有生长因子、某些特殊蛋白、功能基团及其它营养物质的细胞外基质，影响和调节细胞的粘附、增殖、迁移、分化以及死亡等细胞功能。目前对食道上皮基膜的微观构造还不是很清楚，文献上也很少见到相关的详细报道。本申请在对食道中支持上皮细胞生长的基膜的蛋白质组成和微观结构进行系统研究的基础上，利用本课题组已经建立的电纺织系统和技术，采用可生物降解吸收的聚合物材料设计和制备类似于食道基膜构造的多孔纳米纤维支架，并对此支架进行生物活性因子接枝及原代上皮细胞种植，以研究体外重建食道上皮的可行性。本申请是建立在我们以前对食道组织工程研究的基础上，不仅为食道组织工程学的研究补充新的内容，对于提高我国在食道组织工程领域的研究水平，在国际研究领域中占有一席之地也具有积极作用。

中文关键词： 食道上皮；基膜；结构分析；体外构建；电纺织

英文摘要： Basement membrane is a porous fiber sheet with nanometer pore size. It located between epithelial cell and connective tissue to support cell growth. Basement membrane is also extracellular matrix comprising specific proteins and functional groups and contain a reservoir of growth factors and other trophic agents that modulate cell function such as adhesion, proliferation, migration, differentiation and so on. However, till now, the constitution and microstructure of the basement membrane in esophageal epithelium has not been studied very clearly. There is little report in literatures about this issue. Our group proposes to systemically study about the protein components and micro-topography of the esophageal basement membrane. On the base of this fundamental research, biodegradable porous scaffold will be designed to simulate the constituent of esophageal basement membrane using electrospinning technology which has been set up in our laboratory. Furthermore, biomacromolecules like protein or growth factors will be grafted via chemistry technique on the scaffold in order to finally reconstitute esophageal epithelium in vitro. With the technique and research experience about the esophageal tissue engineering, we believe that we will do a good job in this project to extend the knowledge of tissue engineering and upgrade our general research rank in the field of esophageal tissue engineering.

英文关键词： esophageal epithelium; basement membrane;structural analysis; in vitro construction; electrospinning