钙镁硅系生物陶瓷溶出离子对骨再生相关生长因子表达的促进效应及其对骨组织再生的调控

项目名称： 钙镁硅系生物陶瓷溶出离子对骨再生相关生长因子表达的促进效应及其对骨组织再生的调控

项目编号： No.51202151

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 无机非金属材料学科

项目作者： 陈显春

作者单位： 四川大学

项目金额： 25万元

中文摘要： 骨再生相关生长因子对构建生物活性组织工程骨起着关键的作用，但可溶性生长因子存在易失活、潜在毒性和剂量依赖性以及需反复手术补充等实际应用障碍。研究表明钙镁硅系生物陶瓷的溶出离子能够促进骨组织细胞增殖、分化及相关基因表达，在体内外均能刺激骨和血管再生。本研究在分析不同浓度的钙、镁、硅离子及离子组合对骨组织细胞代谢、血管生成相关生长因子及基因表达的显著性及协同效应并探索其作用机理，找出骨再生所需离子的临界浓度及促进新骨生长、血管化相关生长因子及基因表达的最佳离子组合的基础上，参考相图，调整CaO、MgO、SiO2三者比例，设计制备出通过逐渐释放一定比例的钙、镁、硅离子而对骨再生具有诱导效应的新型钙镁硅系陶瓷。探索其离子释放动力学特性与组分之间的关系、体外生物活性功能及其在体内对骨再生修复治疗的效能和机理等，为设计制备出离子释放动力学及生物学响应可控的新型骨组织支架材料提供实验依据和理论基础。

中文关键词： 钙镁硅系陶瓷；骨再生；材料设计；组织工程骨；分子生物学

英文摘要： Bone-related growth factors play a crucial role in building bioactive tissue-engineered bone. Nevertheless, the soluble growth factors present some disadvantages in clinical application, including easy inactivation, potential toxicity and dose-dependent manner and the need for repeat surgery supplement. It has been reported that the ionic products released from CaO-MgO-SiO2-based bioceramics could promote cell proliferation, differentiation and expression of bone tissue related genes, and stimulate bone and vascular regeneration in vivo and in vitro. The cooperative effects of different concentrations of Ca, Mg, Si and the ion combination on cell metabolism, angiogenesis, expression of bone-related growth factors and gens as well as the reaction mechanism are studied in this research, and then the critical concentrations of different ions for bone regeneration and the optimal ions combination stimulating new bone growth, vascularization and expression of bone regeneration-related growth factors and genes are found. On this basis, according to the CaO-MgO-SiO2 ternary phase diagram, through adjusting the ratio of CaO, MgO and SiO2, a novel CaO-MgO-SiO2-based bioceramics which could release a certain concentration rang of Ca, Mg，Si ions to induce bone regeneration can be designed and synthetized. The relationship

英文关键词： CaO-MgO-SiO2-based ceramics；bone regeneration；design of material；tissue-engineered bone；molecular biology