一体化3D喷墨生物打印构建预制血管网络系统的组织工程骨

项目名称： 一体化3D喷墨生物打印构建预制血管网络系统的组织工程骨

项目编号： No.81501879

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 段鑫

作者单位： 四川大学

项目金额： 18万元

中文摘要： 通过长期研究，体外构建的组织工程骨已进行过大量的体内植入试验。但是，现有的组织工程骨内没有现成的血管网，同时也没有组成血管的细胞，在体内血管化程度有限，是阻碍组织工程骨应用的最大障碍之一。移植物的血管化是骨组织再生成功与否的决定因素。生物活性骨的快速血管化问题，成为骨再生和骨修复研究的重要课题。针对血管化问题，申请者提出在体外一体化构建组织工程骨及其内部血管系统的设想。本研究采用一体化3D喷墨生物打印技术，构建预制血管网络的组织工程骨。在体外预制好血管相及骨相的生物墨水，再一体化3D打印组织工程骨。血管相中的内皮细胞可发展成为类似中央血管，骨相中种植的内皮细胞和成骨干细胞可以诱导成类似交通血管网及骨质。申请者掌握的程序化诱导技术让这一设想成为可能。本研究将采用全新一体化3D喷墨生物打印技术构建预制血管网络组织工程骨，并了解其修复大段骨缺损疗效，为以后临床治疗骨缺损提供新的思路及理论依据。

中文关键词： 骨缺损；血管网；3D；打印；组织工程骨；骨修复

英文摘要： After long time research, there are plenty of implantation experiments of tissue engineered bone. However, there were no blood vessels or cell component of blood vessels in the existing tissue engineered bone. The limitation of vascularization is one of the key problems to keep tissue engineered bone from applying in clinic. Vascularization is a critical factor for tissue engineered bone alive and conduct its function. To vascularize the tissue engineered bone becomes the key point of bone regeneration and bone repair research. We plan to construct a bone scaffold with blood vessels in this project. Intergrated 3D inkjet bioprinting technique make this come ture. Prefabricated bioink of blood vessels phase and bone phase，tissue engineering bone was printed integratly. The vessel phase containing endothelial cell can be induced to central blood vessels, while the bone phase containing endothelial cell and osteoblastic stem cell can be induced to traffic blood vessels and osteoid tissue by programmatic differentiation technique. Intergrated 3D inkjet bioprinting technique is a novel technique for tissue engineering. Tissue engeering bone with blood vessels will be systematicly investigated and the effect of repairing bone defect will be studied. The achievement will provide experimental evidences and example for clinic applying of tissue engineered bone.

英文关键词： bone defect;blood vessel net;3D printing;tissue engineering bone;bone repair