水孔蛋白在硅诱导番茄抗盐、抗旱中的作用机制研究

项目名称： 水孔蛋白在硅诱导番茄抗盐、抗旱中的作用机制研究

项目编号： No.31272152

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 宫海军

作者单位： 西北农林科技大学

项目金额： 80万元

中文摘要： 硅能提高植物对各种胁迫的抗性，但其作用机理尚不清楚。前人及我们前期研究表明，硅可调控盐胁迫和干旱下植物根系的水分吸收、改善植物水分状况、提高植物的抗性。水孔蛋白(aquaporin, AQP)是根系水分吸收的主要途径，但对其在硅诱导植物抗盐抗旱中的作用机理尚不清楚，国内外尚无报道。本项目拟在前期工作基础上，以不同抗性番茄品种为材料，应用压力探针、real-time PCR等技术，阐明盐胁迫和干旱下AQP在硅调控番茄水分吸收运输中的作用、确定起关键作用的AQP；克隆硅转运体Lsi-1和关键AQP基因，构建硅转运体基因的RNA干扰与过表达、及关键AQP的RNA干扰遗传转化体系，获得转基因株系，研究AQP在水分吸收运输中的功能及硅的调控；利用基因芯片分析硅调控AQP所涉及的代谢途径和关键因子。通过以上研究，阐明AQP在硅诱导番茄抗盐抗旱中的作用机制。此研究将为硅肥在蔬菜生产中的应用提供理论依据。

中文关键词： 番茄；抗盐抗旱性；硅；水孔蛋白；作用机制

英文摘要： Silicon can increase the tolerance of plants to various environmental stresses. However, the mechanisms are still unclear. Previous research by us and other researchers has indicated that silicon can regulate water uptake by roots, improve water status of plants, and increase plant tolerance under salt and drought stress conditions. Aquaporin (AQP) is the main pathway of root water uptake in plants. However, the mechanisms for the involvement of aquaporin in silicon-induced salt and drought tolerance of plants are still not clear, nor have these been reported. Based on our previous research, the following work will be conducted in this project using tomato cultivars with different tolerance to salt and drought stresses: (1) clarifying the roles of AQPs in the regulation of root water uptake of tomato under salt and drought stress by using pressure probe, real-time PCR，and routine plant physiological techniques, and determining the key AQP; (2) verifying the function of AQPs and the regulative role of silicon in root water uptake and transport by using RNA interference (RNAi) and overexpression transgenic plants of silicon transporter gene Lsi-1, and RNAi transgenic plants of the key AQP; (3) studying the metabolic pathway and key genes in the AQP regulation by silicon using gene chip. Through the above research,

英文关键词： Tomato；Salt and drought tolerance；Silicon；Aquaporin；Mechanism