针刺心包经心经抗心肌缺血效应相似性研究

项目名称： 针刺心包经心经抗心肌缺血效应相似性研究

项目编号： No.30873306

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 生物科学

项目作者： 周美启

作者单位： 安徽中医药大学

项目金额： 31万元

中文摘要： 本项目组在分析中医、针灸古代文献及现代研究成果等基础之上，明确提出"心主二经"假说，并引入系统论和相似科学理论认识"心主二经"的内涵：心经-心脏系统与心包经-心（心包）脏系统之间具有很大的相似性。以电针心经、心包经干预急性心肌缺血作用及其机制比较研究为切入点，通过复制大鼠急性心肌梗死模型，观察心电图J点、T波，心肌钙蛋白T，以及心肌梗死面积等指标，比较研究针刺肺经、心包经和心经干预急性心肌缺血作用的异同，以证实针刺心包经、心经干预心肌缺血作用的相对特异性及其抗心肌缺血效应的相似性，并采用基因芯片技术，观察电针心经、心包经对心肌缺血大鼠心脏基因表达谱的影响，比较研究电针心经、心包经干预急性心肌缺血作用的分子生物学机制，分析和筛选出针刺心经、心包经干预急性心肌缺血作用的相对特异性基因群。从器官、组织和分子水平验证"心主二经"假说，并为针灸临床应用提供实验依据。

中文关键词： 手厥阴心包经；手少阴心经；心脏；针刺效应；相似性

英文摘要： The research group raises and establishes the hypothesis "the heartgoverning two meridians" on the basis of anlysis of relationship among the Pericardium Meridian of Hand-Jueyin(PC),the Heart Meridian of Hand-Shao-yin(HT) and the heart by reviewing ancient literature and modern research achievements of TCM, acupuncture and moxibustion ,and further recognizes its scientific connotation at an angle of theories of systems and similarity, namely strong similarity between HT-heart system and PC-heart(pericardium) system. The research starts with anti-myocardial ischemia effects of acupuncture by reconstructing model of acute myocardial ischemia in rats and observing effects of acupuncturing HT or PC on J points and T waves of ECG, cTnT, and myocardial infarction area, etc.,so as to affirm the similarity of anti-myocardial ischemia effects of acupuncturing HT or PC. Furthermore, a comparative study on the above-mentioned experiment is done to find common and differential expression genes by applying gene chip technique in order to look for a group of relatively special genes that may be material basis of two system similarity. The research results will test and verify the hypothesis "the heart governing two meridians" in three levels of organ, tissue and molecule, and also provide experimental basis for clinical application.

英文关键词： Pericardium Meridian of Hand-Jueyin;Heart Meridian of Hand-Shao-yin;Heart;Acupuncture effect;Similarity