雌雄同体石斑鱼性腺发生与性别分化的分子机制

项目名称： 雌雄同体石斑鱼性腺发生与性别分化的分子机制

项目编号： No.U1401213

项目类型： 联合基金项目

立项/批准年度： 2015

项目学科： 管理科学

项目作者： 刘晓春

作者单位： 中山大学

项目金额： 223万元

中文摘要： 石斑鱼类是我国南方重要的海水养殖鱼类。与其他脊椎动物不同，石斑鱼类属于典型的雌雄同体雌性先熟种类，在其生活史中性别存在由雌转雄的性逆转现象。因此，石斑鱼是研究鱼类性别决定与性别分化分子机制的理想模型，但也给石斑鱼类优良品种的遗传改良带来很大困难。本项目以斜带石斑鱼为研究对象，在斜带石斑鱼全基因组图谱完成的基础上，通过研究斜带石斑鱼原生殖细胞发生、迁移和分化的原发性性别分化过程，以及由雌转雄的次发性性别分化过程的转录组和全基因组甲基化的动态变化，筛选重要的调控基因，阐明其在原发性与次发性性腺发生与性别分化调控过程的分子作用机理，从而构建斜带石斑鱼性腺发生与性别分化的分子调控网络，为石斑鱼类育种和遗传改良提供理论基础。

中文关键词： 性腺发生；性别分化；分子机制；雌雄同体鱼类；石斑鱼

英文摘要： Groupers are economically important mariculture species in South China. Different from other vertebrates, groupers are protogynous hermaphrodite species undergoing sex reversal from female to male during their life history. Therefore, groupers become good model for study of sex determination and sex differentiation. The special reproductive physiological characteristics of grouper fish reflect the diverse reproductive strategies in vertebrates, however, it increase the difficulties for the genetic improvement in this species. In the present project, orange-spotted grouper (Epinephelus coioides) is employed as experimental model to unveil the mechanisms of gonadogenesis and sex differentiation, based on the whole genome sequence of this species. Firstly, the patterns of transcriptome and the genomic methylation during the course of primary sex differentiation and secondary sex differentiation will be studied. Subsequently, the differentially expressed genes will be isolated, and their functions involved in the regulation of primary sex differentiation and secondary sex differentiation be elucidated. Finally, based on the studies above, the molecular regulatory networks of gonadogenesis and sex differentiation will be constructed in orange-spotted grouper. Our study will not only be useful for reveal the molecular regulatory mechanisms of hermaphrodite species in vertebrates, but also provide the valuable reference for the selective breeding and genetic improvement in groupers.

英文关键词： gonadogenesis ;sex differentiation ;molecular mechanism ;hermaphroditic fish;grouper