结合关联分析和连锁作图定位小麦赤霉病抗性基因

项目名称： 结合关联分析和连锁作图定位小麦赤霉病抗性基因

项目编号： No.31301306

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 朱展望

作者单位： 湖北省农业科学院

项目金额： 23万元

中文摘要： 赤霉病是世界范围内小麦主要病害，我国长江中下游地区是该病常发、重发区域。随着全球性气候变暖、耕作制度以及耕作方式的改变，小麦赤霉病正向黄淮麦区、北方麦区、西南麦区和西北麦区扩展。利用寄主抗性是控制该病主要手段之一。鉴定小麦赤霉病新抗源和抗病基因、丰富小麦赤霉病抗性的遗传多样性，对提高抗赤霉病小麦育种水平十分重要。 本项目将利用Genotyping by sequencing（GBS）标记、微卫星标记（SSR）对一个包含240个品种（系）的自然群体和一个双单倍体（DH）群体（169个DH家系）进行赤霉病抗性的全基因组关联分析和连锁作图，并通过对比关联分析中与抗赤霉病显著关联的GBS标记/单元型和连锁作图定位的抗赤霉病位点分子标记之间的连锁不平衡关系，进一步解析小麦赤霉病的遗传基础，发现新的赤霉病抗源、QTL及其紧密连锁的分子标记，为更好地进行小麦抗赤霉病分子育种提供材料和技术。

中文关键词： 小麦；赤霉病；关联分析；QTL 定位；

英文摘要： Fusarium head blight (FHB) is a devastating fungal disease of bread and durum wheat worldwide. It commonlly and severely happens in the middle - lower reaches of the Yangtze River, such as Hubei Province, Jiangsu Province and Anhui Province. This disease reduces wheat yield, lowers processing qualities, and makes grain not suitable for human and animal consumption. More recently, FHB has been expanding northwards to the Yellow River - Huaihe River Region. The increasing threat of this disease has created more incentive for plant geneticists and breeders to map the resistances and develop varieties that are resistant to this disease. Application of host resistance is an economically and environmentally friendly control method of FHB. In order to map gene loci underlying FHB resistance, a natural population containing 240 bread wheat varieties/lines and a doubled haploid (DH) population (169 lines) were constructed. The natural population consists of 10 FHB resistant checks, 120 varieties/lines from the Yangtze River Region and 40 varieties/lines from Yellow River - Huaihe River Region, 15 synthetic hexaploid wheat lines, 25 synthetic-derivatives and 30 CIMMYT elite lines. The DH population was produced from a FHB resistant synthetic-derived bread wheat line. These materials were planned to be genotyped by genoty

英文关键词： wheat；fusarium head blight；association mapping；QTL mapping；