白藜芦醇通过脂联素调控猪骨骼肌肌纤维类型转化及机制研究

项目名称： 白藜芦醇通过脂联素调控猪骨骼肌肌纤维类型转化及机制研究

项目编号： No.31460606

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 畜牧学与草地科学

项目作者： 黄艳娜

作者单位： 广西大学

项目金额： 52万元

中文摘要： 猪肉品质与调控是动物营养研究领域的热点。肌纤维类型是构成不同品种间肉质差异的关键因素之一。Ⅰ型肌纤维含量高的猪肉肉质优良；Ⅱb 型肌纤维含量高则肉质欠佳。提高I型肌纤维的比例是改善猪肉质的主要途径。脂联素是重要的脂肪细胞因子，前期研究发现它能调控小鼠骨骼肌中Ⅰ型肌纤维比例。白藜芦醇（RES）是自然界中一种植物多酚化合物，近年来研究发现RES能调节脂联素的表达。本项目先以肉质优良的广西陆川猪和肉质欠佳的杜长大猪作为模型，研究二者肌纤维类型差异，脂联素及其受体与肌纤维类型相关基因的相关性；并探讨脂联素调控猪骨骼肌纤维类型的机制；后期利用细胞试验研究RES通过调控脂联素基因表达对猪骨骼肌纤维类型的影响，通过饲养试验研究RES调控猪肉质和肌纤维类型的作用，探讨其应用于猪幼年及生长肥育饲养中适宜的添加比例。本项目为开发RES 作为一种通过调控肌纤维类型进而改善猪肉质的饲料添加剂提供科学理论依据。

中文关键词： 白藜芦醇；脂联素；猪；肌纤维类型；分子机制

英文摘要： Pork quality and regulation has become hot field of animal nutrition research. Muscle fiber type is one of the key factors that constitute the difference between different varieties of meat. Excellent pork meat has high level of type I muscle fiber. Poor meat has high level of type II muscle fiber. Increasing the proportion of type I muscle fibers is the main way to improve pork quality. Adiponectin is one of the important adipocytokines. Previous study in mice found that it can regulate the proportion of type I muscle fiber. Resveratrol (RES) is a non-flavonoid polyphenol compound. Recent study found that RES can regulate the expression of Adiponectin. We study the differences of muscle fiber types between Guangxi Luchuan pigs with excellent meat and Duroc× Landrace ×Yorkshire pigs with poor meat, and correlation of Adiponectin, its receptors and MyHCs; And explore the regulation mechanism of muscle fiber type in pig by Adiponectin; At last study on the effect of RES on porcine skeletal muscle fiber types by regulating the gene expression of Adiponectin. In order to study the effect of RES on regulating pig meat quality and muscle fiber types, we explore the appropriate add rato feeding the pigs at the stage of the childhood and growing-finishing. The study provides important scientific theory of developing RES as a feed additive for improving meat quality by regulating muscle fiber types.

英文关键词： Resveratrol;Adiponectin;pig;muscle fiber tpyes;molecular mechanism