水稻OsSRO1c基因在干旱胁迫诱导的叶片衰老中的功能研究

项目名称： 水稻OsSRO1c基因在干旱胁迫诱导的叶片衰老中的功能研究

项目编号： No.31500223

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 生物科学

项目作者： 游均

作者单位： 中国科学院武汉植物园

项目金额： 20万元

中文摘要： 干旱是全球作物生产最重要的制约因素之一。叶片的寿命与植物非生物逆境抗性紧密相关。研究胁迫条件下水稻叶片衰老的调控机制，对于提高水稻抗逆性，减少非生物逆境胁迫造成的产量损失具有重要理论和应用价值。前期的工作中，项目申请人鉴定了水稻抗逆转录因子SNAC1的一个靶基因OsSRO1c在促进气孔关闭中的作用。最近的研究显示OsSRO1c还特异地参与了干旱胁迫诱导的叶片衰老调控。本项目通过突变体和转基因材料的表型鉴定，结合转录组分析，揭示OsSRO1c在干旱胁迫诱导叶片衰老中的作用机制。通过深入研究OsSRO1c下游基因和互作蛋白的功能，揭示OsSRO1c介导干旱胁迫应答途径中的关键因子和分子网络调控机制。通过本项目的研究，不仅有助于全面解析OsSRO1c在逆境应答中的功能，更丰富了对水稻干旱胁迫应答复杂调控网络的认识，为水稻耐旱遗传改良提供了理论基础和基因资源。

中文关键词： 水稻；干旱胁迫；叶片衰老；蛋白质相互作用；信号传导

英文摘要： Drought stress is one of the most important constraints in crop production around the world. Increasing evidence suggests that plant responsiveness to abiotic stress is closely associated with leaf longevity. Thus, understanding the regulatory mechanism of leaf senescence under abiotic stress conditions in rice has important theoretical and application value for improving rice abiotic stress resistance, and reduces yield loss caused by stresses. In the prophase work, OsSRO1c was identified as one of the target genes of stress related transcriptional factor SNAC1, and have a role in promotion of stomatal closure in rice. This project will further investigate the function of OsSRO1c in drought stress-induced senescence through phenotypic analysis of mutant and transgenic plants, combined with the transcriptomic analysis. Moreover, the function of downstream genes and interaction proteins of OsSRO1c in abiotic stress response will be also analyzed in detail. Thereby the key factors in OsSRO1c-medicated drought stress response pathway will be uncovered. In summary, the function of OsSRO1c in the stress response and the molecular networks of OsSRO1c-mediated drought stress response will be comprehensive analytic in this project. The results of this project will enrich our understanding of the complex regulation network of drought stress response, and provided theoretical basis and genetic resources for genetic improvement of drought resistance in rice.

英文关键词： Rice;Drought Stress;Leaf Senescence;Protein Interaction;Signaling Transduction