大气压等离子体射流促进干细胞增殖与分化的机理研究

项目名称： 大气压等离子体射流促进干细胞增殖与分化的机理研究

项目编号： No.51477066

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 电工技术

项目作者： 卢新培

作者单位： 华中科技大学

项目金额： 90万元

中文摘要： 常温大气压等离子体（RT-APP）在生物医学领域的应用包括灭菌、诱导癌细胞凋亡等。国内外研究者在其机理及应用方面都开展了大量的研究工作。而利用RT-APP来促进神经干细胞的增殖与分化则非常少。本课题将研究RT-APP促进神经干细胞增殖与分化的效果及其机理。通过（1）改变等离子体射流的放电参数，确定他们对神经干细胞增殖、分化的影响效果，获得最优工作条件；（2）测量不同放电参数下等离子体中各种活性粒子浓度，并与第一部分结果进行综合对照分析，初步了解等离子体促进干细胞增殖与分化的机理；（3）对等离子体处理后神经干细胞分化的细胞谱系进行分析，从而深入了解等离子体促进神经干细胞分化的机制；（4）并初步开展等离子体与传统促进神经干细胞分化因子协同作用的研究；及（5）等离子体对神经干细胞增殖、分化作用的生物安全性分析；最后，（6）从动物水平研究等离子体处理对脊髓损伤、脑损伤小鼠的神经系统修复效果。

中文关键词： 气体放电；生物医学应用；低温等离子体；等离子体射流；等离子体诊断

英文摘要： Applications of room temperature-atmospheric pressure plasma (RT-APP) in health care include sterilization, inducing cancer cell apoptosis, etc. Lots of related researches on plasma medicine and the interacting mechanism between plasma and treatment objects have been done by researchers around the world. However, reports about the use of RT-APP to promote the proliferation and differentiation of neural stem cells were rarely seen. In this project, we will investigate the effects of RT-APP on the proliferation and differentiation of neural stem cell and the mechanisms underlying. (1) Through changing the discharge parameters of plasma jet, we will study the effects of RT-APP on the proliferation and differentiation of neural stem cell, and get the optimal working conditions. (2) To get a preliminary understanding of the mechanism on the promoting effect of plasma on the proliferation and differentiation of stem cells, concentrations of various reactive species will be measured under different discharge conditions. (3) We will also analyze the differentiated cell lineages to get a better understanding of the mechanism on the promoting effect of plasma on neural stem cells. (4) Preliminary exploration will be carried out on the synergy of plasma and traditional differentiation promoting factor of neural stem cell. (5) Moreover, biological safety of plasma on neural stem cell will be explored. (6) In the end, the effects of plasma on the nervous system repair of spinal cord injury and brain damage will be also studied from animal level.

英文关键词： gas discharge;biomedical application;low temperature plasma;plasma jet;plasma diagnostic