基于转录组和代谢组的马铃薯抗寒相关途径分析及抗寒机理解析

项目名称： 基于转录组和代谢组的马铃薯抗寒相关途径分析及抗寒机理解析

项目编号： No.31501354

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 食品科学、农学基础与作物学

项目作者： 陈琳

作者单位： 华中农业大学

项目金额： 20万元

中文摘要： 现有栽培的马铃薯品种不抗霜冻，生长期间的寒害对马铃薯生产的造成严重威胁。马铃薯对寒害的抗性包括本身所具备抗寒性和由低温诱导产生的驯化抗性，然而相关机理研究十分缺乏，导致抗寒性状改良成效甚微。本项目拟通过具有不同抗寒水平和抗寒驯化能力的7份马铃薯野生种和1份栽培种，利用转录组学和代谢组学结合的途径，系统研究马铃薯对寒害和低温驯化响应的分子和代谢基础，解析非驯化抗寒和驯化抗寒的调控途径和关键调控因子，以揭示两种抗寒能力形成的分子机制及其相互关系。同时，通过不同遗传背景材料的比较，明确不同野生种抗寒机制上的差异，较全面地解析马铃薯抗寒机理。

中文关键词： 马铃薯；转录组；代谢组；抗寒机理

英文摘要： Current potato varieties are sensitive to frost during plant growth which is a great threat to potato production. Two kinds of cold resistance have been clarified, the non-acclimate resistance (NAR) and low temperature-induced acclimate resistance (AR). However, little is known about their mechanisms, a causal for ineffective improvement of the cold resistance in potato. In this proposed project, seven wild species and one spp. tuberosum cultivar with different level of NAR and AR will be used for systemic analysis of the molecular and metabolic mechanisms underlying potato NAR and AR by an integrated strategy of transcriptomics and metabolomics. The molecular mechanisms of NAR and AR and their relationships could be revealed by clarifying the regulatory ways and the key regulation factors. Meanwhile, comparison between diverse genotypes may offer some insight into the mechanism of potato cold resistance.

英文关键词： Solanum tuberosum;transcriptome;metabolome;mechanism of cold resistance