SsFoxE1转录因子调控核盘菌子实体形成与发育的机制研究

项目名称： SsFoxE1转录因子调控核盘菌子实体形成与发育的机制研究

项目编号： No.31271991

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 潘洪玉

作者单位： 吉林大学

项目金额： 84万元

中文摘要： 核盘菌（Sclerotinia sclerotiorum）是一种不产生无性孢子的植物病原真菌，其中子实体形成与子囊孢子产生是菌核病大发生的前提。项目组前期研究发现：核盘菌SsMADS转录因子参与了核盘菌的有性生殖阶段，并可能调控另一类FoxE1转录因子而起作用。据此，本项目将全面研究核盘菌SsFoxE1的功能，解析其在子实体形成与发育中的调控网络。拟开展工作：（1）构建SsFoxE1突变体，验证SsFoxE1在子实体形成中的生物学特性及对致病性的影响；（2）分析核盘菌野生型与SsFoxE1突变体菌株差异表达谱，克隆SsFoxE1所调控的靶标基因；（3）阐明所调控基因的时空表达动态及与子实体发育之间的关联；（4）针对候选的调控基因及其调控序列，鉴定SsFoxE1所结合的DNA特征序列；（5）阐明SsFoxE1调控机制模型，揭示调控基因与有性生殖之间的关系。为菌核病有效防治提供新思路和新途径。

中文关键词： 核盘菌；Ss-FoxE；转录因子；子实体；调控机制

英文摘要： Sclerotinia sclerotiorum (Lib.) de Bary is a Discomycetes in the class Leotiomycetes necrotrophic fungal pathogen with a broad host range. The causal agent is capable of major infecting soybean, rape, sunflower and so on, causing white mold and stem rot disease, which results in significant yield losses. However, the Sclerotinia sclerotiorum does not produce conidia, but ascospores in the apothecia during growth and development period of its life cycle. And the sexual spores are the primary source of inoculum in susceptible hosts naturally. Our group previously discovered that a typical MADS box transcription factor participates in the sexual developmental process, and probably through regulating a novel transcription factor FoxA1 controls this process in Sclerotinia sclerotiorum. Based on the work above, this project is furthere planning to clarify the SsFoxE1 genes function and deciphering regulating network in fruiting body formation and sexual deevelopment. The research carried out as four respects: (1) to construct SsFoxE1 knockout or knockdown mutant, and identify properties of biology and pathogenicity; (2) to analyze and compare mRNA expression profiles between Ss FoxE1 mutant and wild field type, and clone candidates of the regulated genes; (3) to analyze corresponding relations between the spatial &

英文关键词： Sclerotinia sclerotiorum；Ss-FoxE；Transcription factors；Fruit body；Regulatory mechanism