水稻OsNOX2抗旱的分子机制及其调控信号研究

项目名称： 水稻OsNOX2抗旱的分子机制及其调控信号研究

项目编号： No.31270299

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 陈坤明

作者单位： 西北农林科技大学

项目金额： 78万元

中文摘要： 质膜NADPH氧化酶（NOX）是植物活性氧释放与代谢的关键酶，对植物生长发育调控和环境胁迫响应均至关重要。我们的前期研究表明，水稻NOX酶家族至少有11个成员且其表达具有明显的时空调控特点与逆境响应偏好性；其中OsNOX2基因表达受干旱强烈诱导且一条以OsNOX2为中心的酪氨酸受体蛋白激酶依赖的信号通路被发现可能参与了水稻对干旱逆境的响应，然而OsNOX2抗旱的分子机制尚未阐明，其信号调控途径尚需要更直接的实验证据支持。本项目拟在这些前期研究的基础上，利用相关水稻突变体，通过基因克隆、遗传转化、组织与亚细胞定位表达、蛋白互作分析、蛋白质组学研究、以及理化测定等手段，深入研究OsNOX2抗旱的分子机制及其调控信号通路中几个关键调控节点基因如OsRTPK1、OsRTPK2、OsmtATPS1等的功能，明确这些节点基因与OsNOX2表达之间的关系，阐明OsNOX2抗旱的分子机制及其关键调控信号。

中文关键词： 水稻；活性氧；OsNOX2基因；抗旱性；分子机制

英文摘要： NADPH oxidases (NOXs) are key enzymes in the production and metabolism of reactive oxygen species (ROS),playing vital roles both in plant growth development and stress responses. Previously, we found that at least 11 NOX-encoding genes exist in rice genome and their expressions exhibited obvious spatio-temporal characteristics under various stress conditions, implying their diverse functions in rice stress response and development. Among these NOX genes, OsNOX2 expression was strongly stimulated by drought stress, and an OsNOX2-dependent stress signalling pathway was found to be involved in the stress responses of rice. However, the molecular mechanism for the involvement of OsNOX2 in drought tolerance of rice and its regulatory signalling pathways are still under investigation. In the present project, a series of rice gene knockout mutants as well as a number of molecular and physiological approaches including gene cloning, genetic transformation, gene expression of organization and subcellular localization, protein interaction and proteomic analysis, and physiological measurements will be used for futher investigation to clarify the molecular mechanism of OsNOX2 in rice drought tolerance. Functions of several genes such as OsRTPK1, OsRTPK2, and OsmtATPS1, which might play crucial roles in the OsNOX2-dependent

英文关键词： Rice；Reactive oxygen species；OsNOX2 gene；drought stress tolerance；molecular mechanism