小麦二倍体祖先种胚乳印迹基因的克隆鉴定及功能分析

项目名称： 小麦二倍体祖先种胚乳印迹基因的克隆鉴定及功能分析

项目编号： No.31471479

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 食品科学、农学基础与作物学

项目作者： 辛明明

作者单位： 中国农业大学

项目金额： 87万元

中文摘要： 本申请课题拟以小麦二倍体祖先种乌拉尔图小麦和粗山羊草为材料，利用高通量测序技术，综合采用遗传学、分子生物学及生物信息学多学科交叉手段，从全基因组水平上明确印迹基因的数目、模式及功能类别，分析其在不同小麦二倍体祖先种中的异同，并探讨部分印迹基因在六倍体小麦背景下的印迹模式；在分子生物学水平上，初步查明表观遗传修饰与3-5个印迹基因的表达关系；在上述工作的基础上，进一步采用转基因策略，分析1-2个重要印迹基因的生物学功能，以期为明确印迹基因和种子发育的关系提供重要的参考依据。发表SCI论文1-2篇，培养研究生2-3名。

中文关键词： 小麦；转录组测序；基因印迹；表达分析；功能分析

英文摘要： Wheat (Triticum aestivum）is one of the most widely grown crop in the world, providing approximately 55% carbohydrates for the daily human diet. Genomic imprinting is a form of allele-biased expression involving the uniparental transcription of select genes in a parent-of-origin manner, and it plays a crucial role in plant endosperm development. In this project, we will perform a high-throughput transcriptome sequencing on reciprocal crossed, developing endosperms at 10DAP(days after pollination), 15DAP and 20DAP by using diploid wheat with genome A and D. We will predict candidate imprinted genes on genomic level with bioinformatics pipeline and analyze their dynamic expression pattern during endosperm development. Moreover, to understand the mechanism underlying imprinting, we will investigate their differences on DNA methylation and histone modification level between parental alleles. Additionally, the conservation of imprinting among different species, two diploid wheat as well as three homeologous alleles in hexaploid wheat will be estimated. Based on the previous studies, we hypothesize that gene imprinting is involved in regulating wheat endosperm development, so we will perform functional analysis of 1-2 candidates through transgene method. The proposed research will advance our understanding on the number, expression and evolution pattern of imprinted genes in wheat, and how the imprinted genes influence wheat endosperm development. If this project is financially supported, we will publish 1-2 SCI papers and train 2-3 Ph.D students.

英文关键词： wheat;transcriptome sequencing;gene imprinting;expression analysis;functional analysis