BAM介导的CLE-RLK-WOX信号通路对杨树维管形成层活性的调控机制研究

项目名称： BAM介导的CLE-RLK-WOX信号通路对杨树维管形成层活性的调控机制研究

项目编号： No.31470614

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 王洁华

作者单位： 天津大学

项目金额： 93万元

中文摘要： CLE-RLK-WOX信号通路在植物顶端分生组织中已被证实对干细胞的正常分裂与分化具有决定作用，是近年来植物生长发育领域的研究热点,但CLE-RLK-WOX信号通路在维管分生组织中的研究较为落后，尤其在木本植物中的研究很少。本项目在前期工作中分离到3个杨树BAM基因，表达谱分析和启动子融合GUS标记实验显示它们具有不同的维管组织表达特征。PtBAM基因在拟南芥和杨树中的过量表达时会严重影响维管组织的形态建成和后期发育。本项目以杨树为主要研究对象，通过生物信息学手段分析BAM基因在植物中的系统进化关系；掌握其在模式植物中的基因共表达规律和时空表达特性；利用突变体互补、基因改造和特异启动子控制下的转基因等手段进行功能验证，力求阐明BAM基因在维管形成层CLE-RLK-WOX调控网络中的功能和作用机制,对揭示维管形成层发育的分子调控机制具有重要意义，是对树木生长发育领域相关研究的重要补充。

中文关键词： 杨树；维管形成层；信号通路；植物激素；调控机理

英文摘要： CLE-RLK-WOX signaling pathway has been shown to take a decisive role on the normal division and differentiation of stem cells in plant apical meristems and therefore has become a research hotspot in the area of plant growth in recent years. However, CLE-RLK-WOX signal transduction in vascular meristem has been poorly studied, especially in woody plants. In the preliminary work of this project, 3 poplar BAM genes were separated, and expression profiling analysis and promoter:GUS fusion experiments showed that they have differential vascular tissue-specific expression patterns. When transformed into Arabidopsis and poplar, PtBAM gene overexpression seriously affected the morphogenesis and development of vascular tissue. Taking poplar as the main study object, this project will study the evolutionary phylogenetic relationships of BAM genes in plants by bioinformatics analysis; analyze the temporal and spatial expression of BAM genes in model systems; and verify gene functions through mutant complementation, genetic modification, and the cell-type-specific gene expression transgenic tools. We aim to clarify the regulatory roles and working mechanisms of PtBAM genes in the vascular cambium CLE-RLK-WOX network. This work is of great significance by revealing the molecular mechanism controlling the maintenance and development of vascular cambium, and is also an important complement to the relevant research field of tree growth.

英文关键词： poplar;vascular cambium;signaling pathway;plant hormone;regulation mechanism