“纳米晶面”调控吸附蛋白质功能位点取向的微纳结构表面制备和评价

项目名称： “纳米晶面”调控吸附蛋白质功能位点取向的微纳结构表面制备和评价

项目编号： No.51472216

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 一般工业技术

项目作者： 翁文剑

作者单位： 浙江大学

项目金额： 85万元

中文摘要： 针对生物材料表面微纳结构与细胞相互作用机理核心科学问题，本项目采用TiO2典型原子排列面（极性/非极性面和有序/无序面）形成纳米多面体作为结构单元构建微纳结构表面，首次独特地以纳米结构单元表面－纳米晶面原子排列状态为研究考量切入点，探索其对蛋白质吸附状态以及RGD和RFK序列功能位点取向调控作用。根据纳米晶面～表层水分子状态～吸附蛋白质状态～功能位点取向～干细胞分化关键节点链的探源式思路，揭示表面微纳结构调制干细胞成骨响应性内在规律。采用功能导向式材料设计思想，深入研究RGD和RFK序列功能位点同时合理取向的，易被干细胞感知的，高成骨分化导向的微纳结构表面的构建。本项目主要将开展不同纳米晶面特征的TiO2微纳结构化表面的制备和表征、蛋白质吸附行为和对间充质干细胞响应性影响的三方面研究。本项目为植入体高成骨响应性表面改性提供了新思路和手段，具有重要的理论指导意义。

中文关键词： 微纳结构化表面；纳米晶面；二氧化钛；间充质干细胞的粘附与分化；蛋白质功能位点

英文摘要： For significant key scientific issue of the mechanism on the interaction of micro/nano structured surface with cells, this project intends to adopt nano-sized TiO2 polyhedra with typical atomic arrangement surface (polarity/nonpolar and order/disorder) as structural unit to build micro/nano structured surfaces. Uniquely, in the view of nano-structural unit surface atomic arrangement state-nanosized facet, its influence on functional site orientations of RGD and RFK sequences in adsorbed proteins is explored. An insight understanding of regulation of stem cell adhesion and osteogenesis differentiation by micro/nano structured surfaces will be conducted under considerations in nanosized facets - surface adsorbed water molecules- functional site orientation in adsorbed proteins -responses of stem cells interacting chain. As an idea of functions oriented design, in-depth study on assembling a micro/nano structured surface with simultaneously reasonable orientation of both RGD and RFK sequences, easy perception for stem cells, and high direction to osteogenesis differentiation is conducted. In this project, the preparation and characterization of TiO2 micro/nano structured surfaces with different atomic arrangement characteristics of nanosized facets, protein adsorption behaviors of the micro/nano structured surfaces and the relationship of the micro/nano structured surfaces with responses of mesenchymal stem cells are considered as main research contents. This work provides a new approach of thought and method on surface modification of implants for obtaining highly osteogenesis responses, and makes important theoretical significance.

英文关键词： micro/nano structured surface;nanosized facet;TiO2;adhesion and differntiation of mesenchymal stem cells;protein function sites