生物微结构光响应的调控及DNA分子识别研究

项目名称： 生物微结构光响应的调控及DNA分子识别研究

项目编号： No.51273111

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 一般工业技术

项目作者： 冯传良

作者单位： 上海交通大学

项目金额： 80万元

中文摘要： 目前构建工艺简单、高效而又能灵敏识别生物分子的功能化微纳米结构是生物诊断技术发展中富有挑战性的研究领域。本项目以具有光响应的生物精细微观结构（如蝴蝶）为模板，采用层层静电吸附自组装（LBL）的方法实现多层功能膜（如正、负电荷聚电解质）或含半导体纳米粒子（如量子点CdS）多层膜在微纳米结构中精确的自组装。根据自组装膜折射率可调控的特点，通过改变多层膜的结构和组分，研究多层膜对生物微观结构的光响应调控规律与机理。基于微量物质能够引起光敏性微纳米结构共振峰偏移或增强的原理，探索光响应生物精细微观结构对生物分子（如脱氧核糖核酸DNA）的识别机理及灵敏性、选择性。该研究有望使生物微观结构保持原有结构的同时出现新的功能，为探索价格低廉、工艺简单、高效而又光敏性的生物分子识别新体系提供了理论依据。

中文关键词： 生物微结构；自组装；分子识别；光响应；细胞检测

英文摘要： The sensitive and selective detection of biomolecules (e.g. nucleic acids) is important for clinical diagnostics, detection of genetic disorders,analysis of food and environmental pollutants, and homeland security.However, it is still big challenge to construct the simple, efficient, and sensitive biofunctional micor- or nanostructures for developping novel biosensors. In this proposal, bio-hiberarchy structure with light-responsive property will be selected as templates. The precise self-assembly of multi-functional layers (e.g. polyelectrolytes) or multi-layers containing semi-conductive nanoparticles (e.g. quantum dots) into the template are carried out. Based on the changes of the refractive index of the template by the introduction of multi-layers, the light-responsive mechanism in the template will be investigated. Since tiny amount materials can already induce the shift of resonance peak or enhancement of bio-hiberarchy structure, the sensitive and selective detection of nucleic acides (DNA) will be performed by using the bi-hiberarchy structure. The study is expected to make the microstructure keep original structure and have new function at the same time. This can provide the theory bases for exploring the cheap, simple technology, efficient and photosensitive system for biological molecules recognizati

英文关键词： biomicrostructures；self-assembly；biorecoginzation；photoresponsive；cells detection