核盘菌转录因子Ss-Nsd1对其小孢子产生与子实体发育的调控机制研究

项目名称： 核盘菌转录因子Ss-Nsd1对其小孢子产生与子实体发育的调控机制研究

项目编号： No.31471730

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 潘洪玉

作者单位： 吉林大学

项目金额： 87万元

中文摘要： 核盘菌（Sclerotinia sclerotiorum (lib.) de Bary）是一种死体营养型植物病原真菌。在生活史中产生小孢子与子囊孢子。子囊孢子是该菌主要初侵染源，而小孢子的产生与功能鲜见报道。前期研究发现，Ss-Nsd1是调控核盘菌小孢子产生与子实体发育的关键转录因子。其突变株产生小孢子、不形成产囊体和产囊丝及子囊孢子。推断Ss-Nsd1是小孢子产生与子实体发育的分子开关。为此，项目拟：（1）验证Ss-Nsd1与小孢子及子实体形成的关系，明确其生物学功能；（2）通过差异表达谱、CLIP-seq与互作分析，阐明Ss-Nsd1所调控的靶标基因，及其调控元件等功能；（3）解析Ss-Nsd1在小孢子和子实体发育中，与靶标基因及相关因子关联，明确其调控途径与调控网络；（4）揭示Ss-Nsd1转录因子对核盘菌小孢子与子实体发育的调控机制。项目旨为深入解析核盘菌无性繁殖与有性生殖奠定基础

中文关键词： 核盘菌；转录因子；小孢子；子实体；调控机制

英文摘要： Sclerotinia sclerotiorum (Lib.) de Bary is a Discomycetes in the class Leotiomycetes necrotrophic fungal plant pathogen with a broad host range. The causal agent is capable of major infecting soybean, rape, sunflower and so on, causing white mold and stem rot disease, which results in significant yield losses. However, the Sclerotinia sclerotiorum does produce microconidia and ascospores in the life cycle. The microconidia，spermatia, is reported very little about production and function. And the sexual spores are the primary source of inoculum in susceptible hosts naturally. We have found that the Ss-Nsd1 is a novel kind of transcriptional factor to regulate microconidia reproduction and fruiting body davelopment passway as molecular switching on the base of our group previously discovery in Sclerotinia sclerotiorum. Based on the work above, this project is furthere planning to clarify the Ss-nsd1 gene function and deciphering regulating network in microconidia formation and fruiting body formation. The research carried out as four respects: (1) to construct Ss-Nsd1 knockout or knockdown mutant, and identify properties of biology and pathogenicity of the Ss-nsd1 mutants; (2) to analyze and compare mRNA expression profiles between Ss-nsd1 mutant and wild field type, and clone candidates of the regulated genes; (3) to analyze corresponding relations between the spatial and temporal dynamic and the stages of microconidia formation and fruiting body development;(4) to identify and confirm candidates of the regulated genes, and its egulated core sequence of the DNA binding motif of the Ss-Nsd1; (5) to exploit related network of the target genes with microconidium reproduction and fruiting formation development, and imagine the model of Ss-Nsd1 transcriptional regulation mechanism. The goal of this project is to reveal the regulation network through Ss-Nsd1 transcription factor, and to clarify the relationship between regulated genes and sexual process, and hereby provide novel idears for control sclerotinia disease of plant crops in the fields.

英文关键词： Sclerotinia sclerotiorum;Transcriptional factor;Microconidia;Fruiting body;Regulating mechanism